Influence of Saponins from Root Bark of  by Different Cutting Method

CUI Chun-li; Wang Min; GUO Dong-yan; TANG Zhi-shu; LI Jin; JI De-fu

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Influence of Saponins from Root Bark of by Different Cutting Method

更新时间：2024-01-04

- Influence of Saponins from Root Bark of by Different Cutting Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 22, Pages: 9-11(2011)
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- Published：2011
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CUI Chun-li, Wang Min, GUO Dong-yan, et al. Influence of Saponins from Root Bark of by Different Cutting Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 9-11.
DOI：

CUI Chun-li, Wang Min, GUO Dong-yan, et al. Influence of Saponins from Root Bark of by Different Cutting Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 9-11. DOI：

摘要

目的: 研究不同切制法对楤木根皮中皂苷类成分的影响。方法: 采用鲜切法和干切法对楤木根皮进行切制

以属性较强的显色法进行总皂苷含量测定

并以HPLC测定皂苷元齐墩果酸的含量。结果: 齐墩果酸在2.08~10.4 mg·L-1线性关系良好( r =0.999 5)

平均回收率98.33 %

RSD 0.83 %。鲜切与传统切制根皮总皂苷平均质量分数分别为8.26 %

6.59 %;鲜切与传统切制根皮齐墩果酸质量分数分别为5.26 %

5.23 %。结论: 楤木根皮采用鲜切法优于传统切制法

能有效降低皂苷类成分的损失

为今后将楤木开发应用提供参考。

Abstract

Objective: To study on effect of saponins from root bark of Aralia chinesis with different cutting methods. Method: Using fresh cutting method and dry cutting method to cut root bark of A. chinesis

determinated total saponins by spectrophotometry which is a specificity for saponins. At the same time

determinated the content of oleanolic acid by HPLC. Result: Regression equations revealed good linear relationships between peak-area ratios and concentrations. Oleanolic acid had good linearity in the range of 2.08-10.4 mg·L-1( r =0.999 5)

average recovery was 98.33 %

RSD 0.83 %( n=6 ). The content of total sapnins from root bark of A. Chinesis with fresh-cut method was 8.26%

dry cutting was 6.59%.The content of oleanolic acid was 5.26 % and 5.23 % respectively. Conclusion: Root bark of A. chinesis using fresh-cut method was superior to conventional dry cutting method

it could effectively reduce losses of saponins component

it could supply reference for development and utilization of A. chinesis in future.

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