Objective: Studying changes of main flavone ingredients in Radix Scutellariae after compatibility with Rhizoma Anemarrhena; determining the amount of main flavone ingredients in the decoction. Method: A Kromasil C18 column (4.6 mm×250 mm

5 μm) was used with a mobile phase of methanol-acctonitrile-0.2% H3PO4 in gradient elution. The flow rate was 1 mL ·min-1

the detection wavelength was at 276 nm and the column temperature was maintained at 30 ℃. Result: The linear range of baicalin

wogonoside

baicalein and wogonin were 9.76-195.2 (r=0.999 2)

3.960-79.20 (r=0.999 9)

1.600-32.00(r=0.999 5)

1.536-30.72 mg ·L-1 (r=0.999 9) respectively. The average recoveries (n=9) of the four components were 96.8%-103.0%

RSD<1.7%. Conclusion: Rhizoma Anemarrhenae combination with Scutellaria Baicalensis have important impact on the main flavone ingredients in Radix Scutellariae

which made the four flavone ingredients increasing significantly.