Preliminary Investigation on Glaucocalyxin A-induced Cytotoxicity in K562 Cells

YANG Wen-hua; ZHANG Jian; GAO Li-wen; WANG Jian-wen

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Preliminary Investigation on Glaucocalyxin A-induced Cytotoxicity in K562 Cells

更新时间：2024-01-04

- Preliminary Investigation on Glaucocalyxin A-induced Cytotoxicity in K562 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 19, Pages: 181-184(2011)
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- Published：2011
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YANG Wen-hua, ZHANG Jian, GAO Li-wen, et al. Preliminary Investigation on Glaucocalyxin A-induced Cytotoxicity in K562 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 181-184.
DOI：

YANG Wen-hua, ZHANG Jian, GAO Li-wen, et al. Preliminary Investigation on Glaucocalyxin A-induced Cytotoxicity in K562 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 181-184. DOI：

摘要

目的: 探讨活性氧(reactive oxygen species

ROS)在蓝萼甲素(glaucocalyxin A

GLA)诱导的人慢性粒细胞白血病K562细胞毒性中的作用。方法: GLA(0.313

0.625

1.250

2.500

5.000

10.000 mg·L-1)处理K562细胞12

48 h后

噻唑蓝(MTT)法观察细胞毒作用;流式细胞术检测GLA(2.5

5.0

10.0 mg·L-1)对K562细胞周期的阻滞作用;流式细胞术检测5.0 mg·L-1 GLA作用后K562中的ROS变化;利用MTT法检测抗氧化剂氮乙酰半胱氨酸(N-acetylcysteine

NAC)对GLA诱导下K562细胞毒作用的影响;流式细胞术检测NAC对GLA诱导下K562细胞周期的影响。结果: GLA对K562细胞的生长抑制呈明显的剂量和时间依赖关系

48 h的IC50分别为6.168

2.968

1.086 mg·L-1;GLA对K562细胞周期的阻滞作用主要发生在G0/G1期;5.0 mg·L-1 GLA作用K562细胞2 h后ROS较空白组上升1.3倍;NAC对GLA细胞毒作用有明显的抑制作用

且随NAC浓度上升

抑制作用增强;NAC能减弱GLA对K562的细胞周期阻滞作用。结论: GLA对K562细胞具有细胞毒及细胞周期阻滞作用

且这些作用与ROS有关。

Abstract

Objective: To investigate the involvement of reactive oxygen species(ROS)in glaucocalyxin A(GLA)-induced cytotoxicity of human chronic myelogenous leukemia K562 cells. Method: After 12

24 and 48 hours treatment by GLA

3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide(MTT)assay was performed to assess the cytotoxicity. The cell cycle was studied by flow cytometry. The ROS burst induced by GLA was also determined by flow cytometry. Result: Exposure of K562 cells to GLA lead to a dose-and time-dependent cytotoxicity with IC50 values of 6.168

2.968

1.086 mg·L-1 for 12

48 h

respectively. The block function of K562 cells cycle after treated GLA mainly occurred on G0/G1 phase. There is a generation of ROS in K562 cell after using GLA. NAC can increase the cell viability and decrease cell cycle arrest. Conclusion: The results suggested that ROS might be involved in the K562 cytotoxicity induced by GLA.

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Related Institution

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The Second Clinical Medical College， Henan University of Chinese Medicine

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