Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin
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Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 22, Pages: 6-8(2011)
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Published:2011
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WANG Yuan-qing, YAN Jian-ye, LUO Kun, et al. Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 6-8.
DOI:
WANG Yuan-qing, YAN Jian-ye, LUO Kun, et al. Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 6-8.DOI:
Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin
Objective: To establish purification technology of total alkaloids from skin of Bufo bufo gargarizans by NKA-9 macroporous resin. Method: The content of total alkaloid (5-HT) from skin of B. bufo gargarizans was chosen as index
optimized condition of adsorption and desorption of NKA-9 macroporous resin and established purification technology condition of total alkaloids from skin of B. bufo gargarizans. Result: Optimum purification process conditions were:used NKA-9 macroporous resin
adsorption temperature was 25 ℃
loaded 4 times the amount of column volume with pH of sample solution was 9;adsorption time was 8-10 h
firstly eluted with 6 times water of column volume
then eluted with 3 times 70% ethanol of column volume. Conclusion: Optimum process could be used for purification of total alkaloids from skin of B. bufo gargarizans.
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