Ultrasonic-assisted Extraction of Squalene from Glycyrrhizae Radix Et Rhizoma and Analysis of Crude Fat Components

GAO Gui-tao; YANG Min-li; LIANG Xin-hua

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Ultrasonic-assisted Extraction of Squalene from Glycyrrhizae Radix Et Rhizoma and Analysis of Crude Fat Components

更新时间：2024-01-04

- Ultrasonic-assisted Extraction of Squalene from Glycyrrhizae Radix Et Rhizoma and Analysis of Crude Fat Components
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 18, Pages: 5-9(2011)
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- Published：2011
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GAO Gui-tao, YANG Min-li, LIANG Xin-hua. Ultrasonic-assisted Extraction of Squalene from Glycyrrhizae Radix Et Rhizoma and Analysis of Crude Fat Components[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 5-9.
DOI：

GAO Gui-tao, YANG Min-li, LIANG Xin-hua. Ultrasonic-assisted Extraction of Squalene from Glycyrrhizae Radix Et Rhizoma and Analysis of Crude Fat Components[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 5-9. DOI：

摘要

目的: 确定甘草中角鲨烯的超声波辅助最佳提取条件

并分析甘草粗脂肪的组成。方法: 分别研究了料液比、浸泡时间、超声温度、超声时间对角鲨烯得率的影响

采用L9(34)正交试验

对提取工艺进行优选;并采用气相色谱-质谱法(GC-MS)分析了甘草粗脂肪的组成。结果: 甘草角鲨烯的最佳提取工艺为料液比1∶15

浸泡时间10 h

超声时间50 min

超声温度50 ℃;甘草粗脂肪中共分离鉴定出26种成分

角鲨烯含量0.8%。结论: 超声法操作简便

开发和利用甘草粗脂肪中的角鲨烯具有较好的社会效益和经济效益。

Abstract

Objective: To determine optimum extraction process of squalene from Glycyrrhizae Radix Et Rhizoma

and analysis of crude fat components. Method: The extraction process was optimized by L9(34) orthogonal design with extraction rate of squalene as index. Four factors were chosen

including ratio of material to solvent

soaking time

ultrasonic temperature and ultrasonic time; And the constituents of crude fat from Glycyrrhizae Radix Et Rhizoma were analyzed by GC-MS. Result: The optimum extraction process was as follows: ratio of material to solvent was 1:15

soaking time was 10 h

ultrasonic time was 50 min

extraction temperature was at 50 ℃; The GC-MS results showed that 26 kinds of compounds were identified

the content of squalene was 0.8%. Conclusion: The ultrasonic method is simple for extracting squalene

and the development and utilization of squalene from licorice crude fat had a better social and economic benefits.

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