Optimization Preparation Process of Small Peptide from Gecko by Bionic Enzymatic Hydrolysis
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Optimization Preparation Process of Small Peptide from Gecko by Bionic Enzymatic Hydrolysis
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 22, Pages: 4-6(2011)
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Published:2011
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LIU Yu-jun, DAI Long, WEI Yong-li. Optimization Preparation Process of Small Peptide from Gecko by Bionic Enzymatic Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 4-6.
DOI:
LIU Yu-jun, DAI Long, WEI Yong-li. Optimization Preparation Process of Small Peptide from Gecko by Bionic Enzymatic Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 4-6.DOI:
Optimization Preparation Process of Small Peptide from Gecko by Bionic Enzymatic Hydrolysis
Objective: To optimize enzymatic hydrolysis technology of Gecko. Method: Folin phenol colorimetry was adopted to determine the content of small peptide
and used it as index
investigated dosage and reaction time of pepsin and trypsin respectively. Result: Optimum technology was as followed:added 5 times water
bioled
kept in room temperature
adjusted to pH 2.0 by diluted HCl
added 2.0% pepsin to medicinal quality
oscillated 2 h at 37 ℃
adjusted pH to 8.0
added 2.0% pepsin to medicinal quality
oscillated 4 h at 50 ℃
kept in room temperature
centrifuged(5 000 r·min-1×5 min)
separated supernatant
microfiltration(0.22 μm)
ultrafiltration(3 000)
collected filtration of relative molecular weight less than 3 000. Conclusion: Optimum enzymolysis process was steady.