Simultaneous Determination of Six Constituents in Flos Lonicerae and Jingqi Jiangtang Tablets by HPLC

YU Jing; DENG Yan-ru; Chen Qi; SONG Li-li; GAO Ying; GUO Li-ping

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Simultaneous Determination of Six Constituents in Flos Lonicerae and Jingqi Jiangtang Tablets by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Six Constituents in Flos Lonicerae and Jingqi Jiangtang Tablets by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 19, Pages: 57-60(2011)
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- Published：2011
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YU Jing, DENG Yan-ru, Chen Qi, et al. Simultaneous Determination of Six Constituents in Flos Lonicerae and Jingqi Jiangtang Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 57-60.
DOI：

YU Jing, DENG Yan-ru, Chen Qi, et al. Simultaneous Determination of Six Constituents in Flos Lonicerae and Jingqi Jiangtang Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 57-60. DOI：

摘要

目的: 建立同时测定金银花及金芪降糖片中6种成分的反相高效液相色潽法。方法: 色谱柱为依利特ODS C18(4.6 mm×250 mm

5 μm);乙腈-0.1%磷酸溶液为流动相

梯度洗脱;流速1 mL·min-1;检测波长355 nm;柱温30 ℃。结果: 6种成分在上述条件下分离良好。绿原酸、咖啡酸、芦丁、金丝桃苷、木犀草苷和4

5-O-双咖啡酰奎宁酸分别在3.24~64.80

0.24~4.79

1.22~24.40

0.09~0.44

0.68~13.55

1.19~23.87 mg·L-1线性良好

平均回收率均大于97%(RSD<3%

n=9)。结论: 本方法操作简单、结果准确、回收率高、重复性好

可用于金芪降糖片及其相关制剂的质量控制。

Abstract

Objective: To establish a high performance liquid chromatography method of simultaneously determining 6 constituents in Flos Lonicerae and Jinqi Jiangtang tablets. Method: The separation was carried out on an ODS C18 column (4.6 mm×250 mm

5 μm) with acetonitrile-0.1% H3PO4 as mobile phase by gradient elution. The flow rate was 1 mL·min-1 and the detection wavelength was at 355 nm. The column temperature was at 30 ℃. Result: The six ingredients were separated well. The linear ranges of chlorogenic acid

caffeic acid

rutin

hyperoside

luteolin-7-O-glucoside and 4

5-O-dicaffeoylquinic acid were 3.24-64.80

0.24-4.79

1.22-24.40

0.09-0.44

0.68-13.55

1.19-23.87 mg·L-1 respectively. The average recoveries were above 97% (RSD<3%

n=9). Conclusion: This method is simple and accurate with good recovery and repeatability

and can be used in Jinqi Jiangtang tablets and the related preparation quality control.

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Related Institution

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