Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC

QIN Ting; Li Ping; ZHENG Yan-chun; WANG Chun-min; ZHANG Rui-hua

您当前的位置：

首页 >

文章列表页 >

Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC

更新时间：2024-01-04

- Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 17, Pages: 78-80(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
QIN Ting, Li Ping, ZHENG Yan-chun, et al. Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 78-80.
DOI：

QIN Ting, Li Ping, ZHENG Yan-chun, et al. Determination of Brucine and Strychnine in Yaotongning Patch by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 78-80. DOI：

摘要

目的: 建立反相高效液相色谱法同时测定腰痛宁贴膏中士的宁和马钱子碱含量。方法: 色谱柱Intersil C8-3(4.6 mm×250 mm

5 μm)

流动相甲醇-水-冰乙酸(20 ∶96 ∶7

用三乙胺调pH为3.10)

流速1.0 mL · min-1

检测波长254 nm

柱温30 ℃。结果: 士的宁在0.020 6~0.123 4 μg线性关系良好(r=0.999 8)

测得平均加样回收率(n=9)为100.28%

RSD 1.33%;马钱子碱在0.013 8~0.083 0 μg线性关系良好

r=0.999 9

测得平均加样回收率(n=9)为99.82%

RSD 0.84%。结论: 方法灵敏、准确、可用于腰痛宁贴膏中士的宁和马钱子碱的质量控制。

Abstract

Objective: To establish the RP-HPLC method for the content determination of brucine and strychnine in Yaotongning Patch. Method: The Intersil C8-3 (4.6 mm×250 mm

5 μm) column was used; the mobile phase consisted of methanol-water-acetic acid (20 ∶96 ∶7)

adjust pH to 3.10 with triethylamine. The flow rate was 1.0 mL ·min-1 and the detection wavelength was at 254 nm; the column temperature was at 30 ℃. Result: The linear range of strychnine was between 0.020 56-0.123 36 μg (r=0.999 8)

the average recovery was 100.28% with a RSD of 1.33% (n=9); the linear range of brucine was between 0.013 84-0.083 04 μg (r=0.999 9); the average recovery was 99.82% with a RSD of 0.84% (n=9). Conclusion: The method is fast

reliable and accurate

it can be used in the quality control of strychnine and brucine in Yaotongning Patch.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Comparison of Quality Change During Processing Process of Achyranthis Bidentatae Radix from Different Origins Based on Color-component Correlation Analysis

Effect of Microemulsion on Content of Index Components in Different Phases of Zexietang Extract

Discussion on Current National Standards for Traditional Chinese Medicine Dispensing Granules

Cloning and Expression Patterns of Coumarate-3-hydroxylase Gene from Angelica sinensis and Its Correlation with Content of Ferulic Acid

Establishment of a Method for Determination of Amygdalin in Quality Control System of Qingfei Paidu Granules

Related Author

DUAN Haohan

JIA Hao

LEI Jingwei

YANG Chunjing

XIE Caixia

GONG Haiyan

ZHU Beibei

WANG Yanjing

Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰