Effects of Sheng-Yu Decoction on Bone Marrow, Cell Cycle and Apoptosis of Myelosuppressed Mice

ZHAO Ju-hua; ZHU Bi-de; HUANG Qian; WANG Pei; QI Guo-hai

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Effects of Sheng-Yu Decoction on Bone Marrow, Cell Cycle and Apoptosis of Myelosuppressed Mice

更新时间：2024-01-04

- Effects of Sheng-Yu Decoction on Bone Marrow, Cell Cycle and Apoptosis of Myelosuppressed Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 16, Pages: 199-202(2011)
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- Published：2011
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ZHAO Ju-hua, ZHU Bi-de, HUANG Qian, et al. Effects of Sheng-Yu Decoction on Bone Marrow, Cell Cycle and Apoptosis of Myelosuppressed Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 199-202.
DOI：

ZHAO Ju-hua, ZHU Bi-de, HUANG Qian, et al. Effects of Sheng-Yu Decoction on Bone Marrow, Cell Cycle and Apoptosis of Myelosuppressed Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 199-202. DOI：

摘要

目的: 观察圣愈汤对骨髓抑制小鼠外周血、骨髓细胞周期和凋亡的影响

并探讨其造血调控的可能机制。方法: 将60只BALB／c小鼠随机分为空白组、模型组、阳性药组、圣愈汤32

8 g·kg-1·d-1 3个剂量组

每组各10只

除空白组外

其他5组用60Coγ照射和ip注射环磷酰胺、氯霉素制备骨髓抑制小鼠模型。造模完成24 h后

空白组和模型组小鼠ig生理盐水

圣愈汤三剂量组分别按含生药32

8 g·kg-1·d-1ig给药

给药体积20 mL·kg-1·d-1;阳性组ip rhG-CSF 125 μg·kg-1·d-1

各组均连续给药7 d。用全自动血细胞分析仪、白细胞计数法、流式细胞术(FCM)分别检测圣愈汤对骨髓抑制小鼠外周血、骨髓有核细胞数、骨髓细胞周期和凋亡率的影响。结果: 圣愈汤32

16 g·kg-1·d-1均能明显升高外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(PLT)和骨髓有核细胞(BMC)数(P<0.05或P<0.01)。模型组、圣愈汤32

16 g·kg-1·d-1组G0/G1期细胞比例分别为(70.38±1.38)%

(62.43±2.03)%

(63.85±2.90)%;S期比例分别为(22.52±1.27)%

(26.35±2.52)%

(24.80±1.48)%;G2/M期细胞比例(7.43±1.04)%

(11.37±2.18)%

(10.95±2.35)%;PI分别为(29.85±1.06)%

(37.66±2.01)%

(35.88±3.13)%;凋亡率分别为(26.88±3.58)%

(19.15±4.30)%

(21.65±4.34)%。圣愈汤32

16g·kg-1·d-1组与模型组比

上述指标有显著差异(P<0.05或P<0.01)。结论: 圣愈汤能改善骨髓抑制小鼠的造血机能

其机制之一可能是通过促进骨髓造血细胞进入细胞增殖周期和抑制其凋亡。

Abstract

Objective: To observe the effect of Sheng-Yu decoction on peripheral blood cells

bone marrow cell cycle and apoptosis in myelosuppressed mice and to explore its possible mechanism in hematopoietic regulation. Method: The 60 BALB / c mice were randomly divided into blank group

model group

positive medicine group

Sheng-Yu decoction

three dose groups (32

8 g·kg-1·d-1)

with 10 mice in each group. Except the blank group. The other 5 groups were established the model of myelosuppression by irradiated 60Coγ and injection of cyclophosphamide and chloramphenicol. After 24 h of the completion of modeling

blank group and model group were ig normal saline

three doses of Sheng-Yu decoction groups were respectively ig corresponding crude drug 32

8 g· kg-1·d-1

with drug volume 20 mL·kg-1·d-1; and the positive control group were injected with rhG-CSF 125 ug· kg-1·d-1. Each group was medicated for 7 days.The effect of Sheng-Yu decoction on peripheral blood cells

bone marrow nucleated cells (BMNCs) and bone marrow cell cycle and apoptosis in myelosuppressed mice was detected by automatic blood cell analyzer

white blood cell counting and flow cytometry (FCM) respectively. Result: Peripheral white blood cells

red blood cells

hemoglobin

blood platelet and BMNCs were increased obviously by Sheng-Yu Granul

groups(P<0.05 or P<0.01). In model group

Sheng-Yu decoction (32

16 g·kg-1·d-1) dose groups

the proportion of G0/G1 phase cells were (70.38±1.38)%

(62.43±2.03)%

(63.85±2.90)%; S phase fraction was (22.52±1.27)%

(26.35±2.52)%

(24.80±1.48)%; G2 / M phase cells (7.43±1.04)%

(11.37±2.18)%

(10.95±2.35)%; PI were (29.85±1.06)%

(37.66±2.01)%

(35.88±3.13)%; apoptotic rates were (26.88±3.58)%

(19.15±4.30)%

(21.65±4.34)% respectively. Compared with the model group

the above parameters of Sheng-Yu Granul

groups were significantly different(P<0.05 or P<0.01). Conclusion: Sheng-Yu decoction could promote erythroid hematopoiesis by accelerating bone marrow cells of proliferation and diferentiation

inhibiting the apoptosis of hemopoietie cells.

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