Objective: To establish an HPLC method for the simultaneous determination of paeoniflorin

geniposide and paeonol in Chaishao Pills. Method: Paeoniflorin

geniposide and paeonol were separated on Inertsil ODS-SP (4.6 mm×150 mm

5 μm) column and detected at 230 nm. The mobile phase was acetonitrile-water with gradient elution. The flow rate was 1.0 mL ·min-1. Result: Paeoniflorin

geniposide and paeonol were linear within the range of 0.494 4-12.36 μg (r=1)

0.492 2-12.305 μg (r=0.999 9) and 0.260 2-6.505 μg

(r=0.999 9) respectively. The average recovery was 98.77% (2.1%)

101.64% (1.9%) and 100.92% (1.7%). Conclusion: The method is simple

reproducible

and it can be used for the quality control of Chaishao pills.