Determination of Rutin from Leaves of  Gathered in Different Areas and Different Periods by HPLC-UV

PEI Xiang-ping; DU Chen-hui; YAN Yan; SHEN Jia-xing; PEI Miao-rong; BAI Yao

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Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV

更新时间：2024-01-04

- Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 21, Pages: 94-96(2011)
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- Published：2011
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PEI Xiang-ping, DU Chen-hui, YAN Yan, et al. Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 94-96.
DOI：

PEI Xiang-ping, DU Chen-hui, YAN Yan, et al. Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 94-96. DOI：

摘要

目的: 考察不同产地及不同采收期酸枣叶中芦丁的含量

以确定其最佳采收期

为酸枣叶的资源开发提供科学依据。方法: 采用HPLC

Diamonsil(钻石)C18色谱柱(4.6 mm×200 mm

5 μm )

流动相甲醇-0.1%冰醋酸(34 ∶66)

检测波长257 nm

流速1.0 mL·min-1

柱温30 ℃。结果: 芦丁在0.221 6~2.216 μg有良好的线性关系(r=0.999 9)

平均加样回收率为99.40% (RSD 2.42%

n=6);酸枣叶中芦丁含量一般以5~6月含量最高

7~8月芦丁含量有所下降

9~10月又有所升高。结论: 酸枣叶中芦丁的含量整体上随时间变化基本呈U字形趋势

该方法准确、简便、稳定

为酸枣叶的质量标准提供参考。

Abstract

Objective: To study the content of rutin from the leaves of Ziziphus jujuba gathered in different areas and different periods for ascertaining the best harvest time and providing rational basis for the resource development. Method: The assay was performed on a Diamonsil C18 (4.6 mm×200 mm

5 μm) column by an isocratic elution with methanol-0.1% acetic acid(34 ∶66) at a flow rate of 1.0 mL·min-1. The detection wavelength was at 257 nm.The column temperature was kept at 30 ℃. Result: Rutin was linear in the range of 0.221 6-2.216 μg (r=0.999 9). The average recovery ( n = 6) was 99.40% (RSD 2.42%). The content of rutin reached its maximum yields in May and June

however

the content drop down in July and August and rise again in September and October. Conclusion: This method was accurate

simple and stable. The content of rutin changing with time showed a U trend. It provided reference for quality evaluation criteria of the leaves of Z. jujuba

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Related Institution

Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine

College of Life Sciences， Beijing University of Chinese Medicine

College of Chinese Materia Medica， Beijing University of Chinese Medicine

College of Pharmacy, Shaanxi University of Chinese Medicine

Guizhou Provincial Engineering Center of Efficient Utilization of Natural Medicinal Resources, High Educational Key Laboratory of Natural Medicinal Pharmacology and Druggability of Guizhou Province, Joint Key Laboratory of Guizhou Medical University-Guiyang City, Key Laboratory of Optimal Utilization of Natural Medicinal Resources, School of Pharmaceutical Sciences, Guizhou Medical University

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