Effects and Mechanisms of Gexia Zhuyu Decoction on Immunological Hepatic Fibrosis in Experimental Rats
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Effects and Mechanisms of Gexia Zhuyu Decoction on Immunological Hepatic Fibrosis in Experimental Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 22, Pages: 187-190(2011)
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Published:2011
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DAI Qiao-mei, JIA Yan, LIU Hong, et al. Effects and Mechanisms of Gexia Zhuyu Decoction on Immunological Hepatic Fibrosis in Experimental Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 187-190.
DOI:
DAI Qiao-mei, JIA Yan, LIU Hong, et al. Effects and Mechanisms of Gexia Zhuyu Decoction on Immunological Hepatic Fibrosis in Experimental Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 187-190.DOI:
Effects and Mechanisms of Gexia Zhuyu Decoction on Immunological Hepatic Fibrosis in Experimental Rats
Objective: To investigate the mechanisms underlying the prevention effect of Gexia Zhuyu decoction against porcine serum-induced liver fibrosis in rats. Method : Sixty male Sprague-Dawley rats were randomly divided into four groups as follows: blank control group
Gexia Zhuyu decoction group
Porcine serum group
Porcine serum plus Gexia Zhuyu decoction group. The fibrosis model of rats was achieved by intraperitoneal injection of porcine serum twice weekly for 12 weeks
and simultaneously Gexia Zhuyu decoction administered daily by gavage for 12 weeks. All rats were sacrificed at the end of experiment
and their blood and liver were collected for analysis. The liver histopathology was examined by HE staining. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by biochemicalanalysis. MMP collagenase activity in the liver were detected by enzyme linked immunosorbent assay. Result: Compared with the porcine serum group
the histopathology of rats given Gexia Zhuyu decoction improved significantly. ALT and AST in serum and MMP collagenase activity in liver were significantly decreased in porcine serum plus Gexia Zhuyu group (P < 0. 05). Conclusion : Gexia Zhuyu decoction may exert anti-hepatic fibrosis effect in rats via inhibition of hepatic injury and MMP collagenase activity.