Objective: To establish quality control system of suppository for Flos Chrysanthemi Indici (FCI) by identification and content determination. Method: The suppository for FCI was identified by TLC with luteoloside and linarin as indexes. The content of chlorogenic acid and linarin in the suppository for FCI was determined by HPLC. Result: On the silica gel thin layer plate

developed with mixture of acetic ether-acetone-formic acid-water (7∶3∶1∶1) and sprayed with 2% aluminum trichloride ethanol solution

luteoloside and linarin were detected in the suppository for FCI. Chlorogenic acid was separated well in Agilent Eclipse column with isocratic elution by methanol-acetonitrile-0.5% glacial acetic acid (5∶6∶89) and detected at 326 nm. Linarin was separated well in Agilent Eclipse column with isocratic elution by methanol-0.5% glacial acetic acid (47∶53) and detected at 326 nm. Chlorogenic acid and linarin showed a good linear relationship with its peak area in the range of 3.27-408.40 mg ·L-1 and 0.63-79.10 mg ·L-1 respectively. Their average recoveries were 100.16% and 96.99% with RSD of 1.44% and 0.88% respectively. Conclusion: These methods are simple and feasible with good reproducibility. The study will provide a quality control method of suppository for FCI.