Objective: To study the curative effects of autologous bone marrow and umbilical cord blood nuclear cells transplantation treating amyotrophic lateral sclerosis (ALS). Method: The autologous bone marrow of a patient with ALS or umbilical cord blood were collected.Then the nuclear cell suspensions containing stem cells were made. Each suspension contained 4.72×108 nuclear cells averagely. The suspensions were injected into subarachnoid space of the patient by lumbar puncture. In 4 years we performed 3 transplantations of autologous bone marrow nuclear cells and 50 transplantations of umbilical cord blood nuclear cells on the patient respectively. The changes of movement function

cerebrospinal fluid (CSF) and adverse reactions of the patient were observed. Result: The patient’s condition improved after the first transplantation

and to the best after the third transplantation. From then on the patient’s condition worsened gradually

but the worsening speed was slower than before the transplantation. The pressure

routine test and biochemistry indicators of CSF were normal in the 4 years. No severe adverse reactions were observed. Conclusion: Autologous bone marrow and umbilical cord blood nuclear cells transplantation has short-term effect in treating ALS. It can slow down the progression of the disease

and the treatment is safe.