Objective: To observe the effect of Huayu Wan on tumor growth and expression of thrombosis related factors in Lewis lung cancer-bearing mice. Method: Lewis lung cancer model in mice was established. They were then divided into 5 groups 24 h after model establishment:model group (sterile water 10 mL ·kg-1 ig

0.9%NaCl 10 mL ·kg-1 ip

daily)

low-molecular-weight heparins group(sterile water 10 mL ·kg-1 ig

low-molecular-weight heparins 100 u ·kg-1 ip

daily)

cyclophosphamide (CTX) group (sterile water 10 mL ·kg-1 ig

CTX 50 mg ·kg-1(1

6

11

16 d) ip

once)

Huayu Wan group (Huayu Wan 4 g ·kg-1 ig

0.9% NaCl 10 mL ·kg-1 ip

daily) and control group (sterile water 10 mL ·kg-1 ig

0.9%NaCl 10 mL ·kg-1 ip

daily). The tumor inhibiting rate

the inhibiting rate of lung metastasis and change of weight was observed regularly and the levels of tumor necrosis factor-α(TNF-α)

P-selectin

endothelin-1(ET-1) in peripheral blood serum were tested by ELISA. Result: The decrease of tumor weight was obvious in Huayu Wan group (P<0.01). The tumor inhibition rate was 34.97% (13 d) and 31.52% (20 d)

the inhibiting rate of lung metastasis was 83.25% (20 d). The level of ET-1 in peripheral blood serum of Huayu Wan group was lower than those in model group (P<0.05). The level of P-selectin and ET-1 in peripheral blood serum of Huayu Wan group were lower than those in low-molecular-weight heparins group (P<0.05)

the decrease was obvious compared with CTX group (P<0.01). Conclusion: Huayu Wan can be an effective measure for inhibiting the tumor growth and reducing the expression of thrombosis related factor in lewis lung cancer-bearing mice.