Objective : Toxicity of tripterygium wilfordii often bring damage to digestive system

reproductive system

hematological and immune system

so reducing toxicity but maintaining therapy effect has been focused on tripterygium wilfordii studies in the past decade. Combining with traditional Chinese medicine compounds can reduce the toxicity but maintaining therapy effect of tripterygium. Whether Leizhi capsule (Ligustrum lucidum

Eclipta

plus tripterygium glucosides) can reduce toxity and increase effects of Tripterygium glucosides? So this study aims to explore the effects of Leizhi capsule in puromycin amino nucleoside(PAN) nephrosis rats. Method : Eighty male wistar rats were randomly divided into eight groups

including control group

model group

Leizhi capsule high-dosage and low-dosage group

tripterygium glucosides high-dosage and low-dosage group

triptolid group and valsartan group(with each group ten rats). PAN nephrosis medel was made by jugular vein injection of PAN (100 mg·kg-1)

while control group rats were made by injection of physiological saline with equal volume. By irrigation stomach once a day for ten days

all rats had been given medicines as follows: physiological saline 2 mL for control group and model group

tripterygium glucosides 2 mg dissolved in Erzhi(containing Ligustrum lucidum and Drought Ephraim grass) solusion 1 mL or 1 mg dissolved in Erzhi solusion 0.5 mL for Leizhi capsule high-dosage or low-dose group

tripterygium glucosides 10

5 mg·kg-1 for Tripterygium glucosides high-dosage or low-dose group

triptolid 2 mg·kg-1 for triptolid group and valsartan 7.5 mg·kg-1 for valsartan group. The blood and urine samples were collected

liver and kidney tissues were processed after killed. The glomerular morphology and podocyte ultrastructure were observed respectively. Result : In day 5

model rats were in low spirits

with less urine

ascites

malnutrition and weight loss. From day 7 to day 10

the nephrotic syndromes were worst in model rats

but without skin edema. Some rats died of serious ascites

the mortality is 30%(3/10). Morphologic changes include epithelial cells degenerationrenal tubular and transparent cast

but there are no obvious changes in glomerulus and renal interstitial. Meanwhile

the podocyte processes effacement was obvious in model groups in electronic microscope. The nephrotic syndrome of PAN rats treated with Leizhi capsule and tripterygium glucosides significantly improved

compared with that in PAN model group and other treatment groups. The urinary protein excretion was decreased

plasma albumin was increased

and high cholesterol/triglyceride levels were remission

and anemia improved. Serum creatinine increased mildly(P<0.05). In light microscope

epithelial cells degenerationrenal of renal tubular and transparent cast improved in both leizhi capsule and Tripterygium glucosides samples(P<0.05).Meanwhile

the degree and area of podocte processes effacement was significantly reduced in both leizhi capsule and Tripterygium glucosides groups in electronic microscope(P<0.05). Conclusion : Leizhi capsulecan reduce toxity and increase effects of Tripterygium glucosides by reducing proteinuria

improving anemia

lowering AST

improving podocte processes effacement

and reducing epithelial cells degeneration of renal tubular.