Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury
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Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 21, Pages: 168-172(2011)
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Published:2011
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JIA Zhan-hong, ZHAO Hui. Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 168-172.
DOI:
JIA Zhan-hong, ZHAO Hui. Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 168-172.DOI:
Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury
Objective: To investigate the effects of total saponins of Panax japonicus(TSPJ) on neuronal apoptosis and expression of c-fos
c-jun proteins in rat cerebral injury induced by ischemia and reperfusion. Method: Sixty SD rats were randomly divided into 6 groups:control group
model group
nimodipine group
TSPJ 200
100
50 mg·kg-1groups.The models of cerebral ischemia-reperfusion injury was reproduced by middle cerebral artery occlusion(MCAO). HE staining was used to assay the pathological changes;TUNEL stainning was used to detect apoptotic neurons;immuno-histochemistry was used to observe the protein expression of c-fos and c-jun. Result: Compared with the control group
the neuronal apoptosis was increased and the expression of c-fos、c-junhad remarkably enhanced after cerebral ischemia reperfusion(P<0.01);TSPJ improved the pathological changes
TSPJ(200
100 mg·kg-1)group significantly inhibited the expression of postitve neuronal cells of TUNEL and the expression of c-fos
c-jun decreased (P<0.01
P<0.05). Conclusion: TSPJ can decrease the number of apoptotic cells after cerebral ischemia and reperfusion by down-regulating the expression of c-fos and c-jun
which may be one of mechanisms of neuroprotective effects.