Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC
|更新时间:2024-01-04
|
Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 22, Pages: 72-75(2011)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2011
稿件说明:
移动端阅览
CHEN Pei-xin, LI Wei-min, GAO Ying, et al. Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 72-75.
DOI:
CHEN Pei-xin, LI Wei-min, GAO Ying, et al. Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 72-75.DOI:
Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC
检测波长260 nm。 结果: 芒果苷、毛蕊异黄酮苷和毛蕊异黄酮的线性范围分别为36.733~550.999 ng
33.376~166.88 ng
44.544~222.72 ng
相关系数(R2)均>0.999
平均加样回收率分别为102.47%
102.38%
100.20%
其RSD分别为2.23%
2.86%
2.06%。 结论: 方法简便快速
重复性良好
结果准确可靠
可用于知母-黄芪中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮的含量测定。
Abstract
Objective: A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous analysis of mangiferin
calycosin-7-O-β-D-glucoside and calycosin in Anemarrhenae Rhizoma-Astragali Radix. Method: HPLC determination was performed on a Phenomenex Luna C18 (4.8 mm×250 mm
5 μm) and detected at 250 nm. The mobile phase was consisted of acetonitrile and 0.2% phosphate solution with gradient elution. Flow-rate was 1.0 mL·min-1
and column temperature was 30 ℃. Result: The method was proved to be linear in the ranges of 36.733-550.999 ng
33.376-166.88 ng and 44.544-222.72 ng for mangiferin calycosin-7-O-β-D-glucoside and calycosin respectively. The average recoveries were 102.47%
102.38% and 100.20% respectively. Conclusion: The results showed that the method is convenient
accurate and reliable for the simultaneous determination of mangiferin
calycosin-7-O-β-D-glucoside and calycosin in Anemarrhenae Rhizoma-Astragali Radix.
Optimization of Extraction Technology for Total Flavonoids from Diaphragm of
Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Glycyrrhizae Radix et Rhizoma in Qizhiweitong Granules
Structure-activity Omics on Anti-inflammatory and Analgesic Effect of Cyperi Rhizoma in Qizhi Weitong Granules
Analysis of Chemical Constituents in Puerariae Lobatae Radix Dispensing Granules by UPLC-Q-TOF-MS/MS
Effect of Combined Application of Organic Fertilizer and Chemical Fertilizer on Growth, Yield and Quality of Two-year-old Belamcanda chinensis
Related Author
ZHANG Shu-lan
WANG Guo-jun
TANG Hui
LIU Yan-fang
MENG Ying
ZHENG Ying
QIN Xinpeng
LIU Sicong
Related Institution
College of Pharmacy, Liaoning University of Traditional Chinese Medicine (TCM)
Liaoning Multi-dimensional Analysis of TCM Technical Innovation Center
Liaoning Province Modern TCM Research and Engineering Laboratory
China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.
National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine(TCM),Jiangxi University of Chinese Medicine