Objective: To establish a determination method of hesperidin in Nvjin pills. Method: Hesperidin in Nvjin pills was determined by HPLC

using a Shim-pack VP-ODS column(6.0 mm×150 mm

5 μm)

with a mobile phase as methanol-acetonitrile-0.1% phosphoric acid (12∶13∶75)

and a UV detecter at 284 nm wavelength. Result: Hesperidin showed good linearity from 0.02 μg to 4.9 μg (r＝0.999 9). The average recovery of big honeyed pills and water-honeyed pills were 102.0% (RSD 1.3%

n=9) and 96.1% (RSD 1.3%

n=9) respectively. Conclusion: The method was simple

accurate

and with good reproducibility. It can be used well as quality control for Nvjin pills.