Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from  between Microwave-assisted Extraction and Chinese Pharmacopeia Method

FANG Jing; FU Mei-hong; YANG Hong-jun; ZHANG Bei-bei; WANG Zhu-ju; YANG Lan; ZHANG Dong

您当前的位置：

首页 >

文章列表页 >

Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method

更新时间：2024-01-04

- Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 21, Pages: 46-48(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 46-48.
DOI：

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 46-48. DOI：

摘要

目的: 建立微波协助提取法提取黄芩中有效成分黄芩苷

比较微波协助提取法与药典法提取黄芩苷优势。方法: 采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相为甲醇-水-磷酸 (47 ∶53 ∶0.2)

检测波长280nm

柱温30 ℃

流速1 mL·min-1。结果: 微波提取时间15 min

提取温度120 ℃

提取溶剂30%乙醇。黄芩苷在0.059~0.59 μg呈良好线性关系

r=0.999 9。平均回收率99.36%( n =6)。结论: 微波提取法较药典法提取黄芩苷更简便快速、结果准确

为一种符合环保绿色理念的含量测定方法

可用于检测黄芩饮片中有效成分黄芩苷含量。

Abstract

Objective: To establish a microwave-assisted extraction(MAE)method for the extraction of the baicalin from Scutellaria baicalensis. Comparison of the determination of baicalin from S. baicalensis between MAE and Chinese pharmacopeia method. Method: Dikma Diamonsil C18 column(4.6 mm×150 mm

5 μm)was used. The mobile phase of methanol-water-phosphoric acid (47 ∶53 ∶0.2). The temperature of column was 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 280 nm. Result: Microwave extractive time was 15 min

the temperature was 120 ℃

the extraction solvent was 60%ethanol.The calibration curve was in good linearity with in the range from 0.059 to 0.59 μg(r=0.999 9). The average recovery was 99.36% ( n =6). Conclusion: The method is simple

rapid

as well as precise and reliable

and can be used for the determination of baicalin in S. baicalensis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Baicalin in Chaihu Jiangan Capsules by HPLC

Optimization of Processing Technology for by Orthognal Design

Measurement of Content of Baicalin from in Different Habitat by HPLC

Processing Quality of

Determination of Baicalin in Qinxuan Granules by HPLC

Related Author

CHEN Hong-xi

Zhou Jun

WANG Dian-rong

SHANG Qiang

LI Gui-bo

WANG Xin-xin

LIU Xiu-hua

HUANG Qing

Related Institution

Department of Pharmacy,First Affiliated Hospital of PLA General Hospital

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory， Jiangxi Province Key Laboratory of TCM Etiopathogenisis， Jiangxi University of Chinese Medicine

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

The Second Clinical Medical College， Henan University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰