Objective: To establish an High Performance Capillary Zone E1ectrophoresis(HPCE) method to determine the content of berberine hydrochloride

jatrorrhizine hydrochloride and palmatine hydrochloride in sankezhen simultaneously. Method: Fused silica capillary coated (75 cm×50 μm) was used for separation in this study. The mobile phase consisted of 50 mmol ·L- 1 borax solution acetateacetic acid in methanol(2∶1). The applied voltage was 25 kV

and the column temperature was maintained at 15 ℃. The chromatogram was detected at 230 nm. Result: Berberine hydrochloride

jatrorrhizine hydrochloride and palmatine hydrochloride obtained over the range of 0.816 8-32.672 0 mg ·L-1 (r2=0.999 4)for palmatine

the range of 0.852 0-8.520 0 mg ·L-1 for jutrorrhizine

and the range of 0.815 2-8.152 0 mg ·L-1 for jutrorrhizine.The average recoveries were 103.8%(RSD 1.14%)

100.7%(RSD 3.46%)and 100.2%(RSD 2.87%) for berberine hydrochloride

jatrorrhizine hydrochloride and palmatine hydrochloride in sankezhen sample respectively. Conclusion: The method is accurate

simple and rapid. It can be used for the quality control of Sankezhen and providing a experimental basis for development its resources.