Objective: To establish a method of simultaneous determining effective components in Fuyankang pills. Method : The content of paeoniflorin

salvianolic acid B and berberine were determined by HPLC. The analytes were separated using a system of acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate solution containing 0.1% phosphoric acid as the mobile phase

and a linear gradient elution program. The flow rate was 1.0 mL·min-1 and the detection wavelengths of paeoniflorin

salvianolic acid B and berberine were 230

286

265 nm

respectively. Result: The HPLC method was found to be sensitive and linear with the range of 0.077 08-0.770 8 μg for paeoniflorin (r=0.999 7)

0.113 9-1.139 μg for salvianolic acid B (r=0.999 9)

and 0.027 8-0.278 μg for berberine (r=0.999 9)

and the average recoveries for paeoniflorin

salvianolic acid B and berberine were 98.87%

99.31% and 100.65%

respectively. There was great difference in the content of paeoniflorin

salvianolic acid B and berberine in Fuyankang pills from different manufacturers. Conclusion: The method is simple

specific and reproducible for the determination of paeoniflorin

salvianolic acid B and berberine in Fuyankang pills by HPLC.