Analysis of Monosaccharides Composition in Polysaccharide of by Precolumn Derivatization and Capillary Gas Chromatography
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Analysis of Monosaccharides Composition in Polysaccharide of by Precolumn Derivatization and Capillary Gas Chromatography
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 21, Pages: 62-64(2011)
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Published:2011
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LIU Gang, WANG Hui, ZHOU Jian, et al. Analysis of Monosaccharides Composition in Polysaccharide of by Precolumn Derivatization and Capillary Gas Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 62-64.
DOI:
LIU Gang, WANG Hui, ZHOU Jian, et al. Analysis of Monosaccharides Composition in Polysaccharide of by Precolumn Derivatization and Capillary Gas Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 62-64.DOI:
Analysis of Monosaccharides Composition in Polysaccharide of by Precolumn Derivatization and Capillary Gas Chromatography
Objective: To analyze the monosaccharides composition and their molar ratio in Tricholoma matsutake polysaccharide. Method: The monosaccharide units were hydrolyzed by trifluoroacetic acid from polysaccharide in T.matsutake and derivatized with hexamethyldisilane (HMDS) and trimethylchlorosilane (TMCS) to form trimethylsilyl derivative. The capillary chromatographic column HP-50 (0.32 mm×30 m) and FID detector were used in GC determination with inositol as internal standard. Result: Under the optimum conditions
the six standard monosaccharides were in baseline separation within 15 min
T. matsutake polysaccharide was composed of glucose
xylose and galactose with the molar ratio of 14.84 ∶1.42 ∶1.00. Conclusion: The results indicated that this method was effective and quick in analyzing the components of monosaccharides.
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