Study on Chromatography Fingerprints of  by HPLC

GENG Dan; MA Wen-Fang; QIU Qin; ZHEN Han-shen; WEI Liang-hong; JIANG Ling-fang

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Study on Chromatography Fingerprints of by HPLC

更新时间：2024-01-04

- Study on Chromatography Fingerprints of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 22, Pages: 54-56(2011)
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- Published：2011
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GENG Dan, MA Wen-Fang, QIU Qin, et al. Study on Chromatography Fingerprints of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 54-56.
DOI：

GENG Dan, MA Wen-Fang, QIU Qin, et al. Study on Chromatography Fingerprints of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 54-56. DOI：

摘要

目的: 采用HPLC建立广西产桑椹的指纹图谱。方法: 采用Agilent Zobax C18(4.6 mm×250 mm

5 μm)色谱柱;以乙腈(A)-0.1%磷酸(B)为流动相进行梯度洗脱

检测波长360 nm

流速1.0 mL·min-1

柱温30 ℃。结果: 检测了广西不同来源10批桑椹药材

标定了10个共有峰

相似度较高

建立的桑椹指纹图谱稳定性、重复性好。结论: 桑椹HPLC指纹图谱可用于桑椹药材的鉴别及质量控制。

Abstract

Objective: To establish the chromatography fingerprint of Morus alba L. by HPLC. Method: The samples are separated on Agilent Zobax C18(4.6 mm×250 mm

5 μm) with acetonitrile-0.1% phosphoric acid as the mobile phase

gradient eluent

at the flow rate of 1.0 mL·min-1 at 30 ℃

the UV detection is seted at 360 nm. Result: Ten samples of M. alba in different origin of Guangxi was detected and 10 peaks in the chromatogram are common

there is a high similarity

the reproducibility and stability is good. Conclusion: The HPLC fingerprint can be used for identification of M. alba as well as quality control.

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CHEN Ying-hong1

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Related Institution

Guangxi University of Chinese Medicine

Henan College of Traditional Chinese Medicine

Puyang Hospital of Traditional Chinese Medicine

Japan Friendship Hospital,,China

School of Chinese Materia Medica,Beijing University of Chinese Medicine

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