Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD

LI Wen-ting; ZHU Ming; MA Lin-ke; CHENG Yong

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Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD

更新时间：2024-01-04

- Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 22, Pages: 50-53(2011)
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- Published：2011
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LI Wen-ting, ZHU Ming, MA Lin-ke, et al. Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 50-53.
DOI：

LI Wen-ting, ZHU Ming, MA Lin-ke, et al. Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 50-53. DOI：

摘要

目的: 建立桔梗高效液相色谱-蒸发光散射检测器(HPLC-ELSD)指纹图谱的分析方法

研究不同产地桔梗药材的质量。方法: 采用HPLC-ELSD

Welch Materials XB-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%甲酸溶液

梯度洗脱

流速0.8 mL·min-1

柱温30 ℃

ELSD检测器漂移管温度50 ℃

氮气流速1.2 L·min-1。结果: 建立了12批桔梗的HPLC-ELSD指纹图谱

共找到16个共有峰

并确定了桔梗皂苷E

桔梗皂苷D3和桔梗皂苷D。结论: 该方法具有良好的重复性和稳定性

为有效控制桔梗药材的质量提供了依据。

Abstract

Objective: To establish a method of fingerprint analysis for Platycodonis Radix by high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD). Method: HPLC was performed on a Welch Materials XB-C18 analytical column (4.6 mm × 250 mm

5 μm) with gradient elution of acetonitrile-0.1% formic acid at the flow rate of 0.8 mL·min-1 with ELSD detector. The column temperature was maintained at 30 ℃

the temperature of drift tube was set at 50 ℃

and the gas flow (N2) was set at 1.2 L·min-1. Result: The HPLC-ELSD chromatographic fingerprint of chemical constituents was established from the 12 batches of Platycodonis Radix and it had 16 characteristic common peaks. Conclusion: The developed method is repeatable and stable

which is helpful to control the quality of Platycodonis Radix.

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Related Institution

Guangxi Traditional Chinese Medical University

Hunan Provincial Institute for Drug Control

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

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