Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 16, Pages: 67-69(2011)
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Published:2011
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FENG Gai-li, GUO Dong-yan, LI Jin, et al. Study on Fingerprint of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 67-69.
DOI:
FENG Gai-li, GUO Dong-yan, LI Jin, et al. Study on Fingerprint of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 67-69.DOI:
Objective: To establish the fingerprints of Malus hupehensis by HPLC. Method: Hypersil BDS C18 (4.6 mm×250 mm
5 μm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was at 25 ℃ and the detection wavelength was at 287 nm.The detection time was 70 min and the flow rate was 1.0 mL·min-1. Result: There were eleven characteristic peaks indicated in fingerprints. Conclusion: This method is simple and accurate with a good reproducibility. It provides a reference standard for the quality control of M. hupehensis.