Objective: To clone elongation factor-1a (EF-1a) gene cDNA and analyse its expression level of the temperature effect. Method: Elongation factor-1a (EF-1a) gene was cloned through constructing cDNA library of Scutellaria baicalensis

and its characteristics was analysed by bioinformatics method. Its expression level of the temperature effect was analysed by using RT-PCR. Result: The length of elongation factor-1a (EF-1a) gene of Scutellaria baicalensis was 1 672 bp

the open reading box located in 97-1 446 with 449 aminos. It was a typical elongation factor domain.The transcription levels of SbEF1a were significantly improved with high and low temperature processing. Conclusion: 1a (EF-1a) gene of S. baicalensis was a elongation factor

participating in translation of protein. Its expression controled by environmental temperature

and its transcription level is significantly increased after high and low temperature processing.