Effect of Zhisheng Capsule on Cell Apoptosis and Fas,FasL Protein Expression in Rats Model of Esophageal Cancer

WANG Xiang-qi; HAN Qian-qian; WANG Xuan; WANG Jun-tao; WANG Yi-bo

您当前的位置：

首页 >

文章列表页 >

Effect of Zhisheng Capsule on Cell Apoptosis and Fas,FasL Protein Expression in Rats Model of Esophageal Cancer

更新时间：2024-01-04

- Effect of Zhisheng Capsule on Cell Apoptosis and Fas,FasL Protein Expression in Rats Model of Esophageal Cancer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 19, Pages: 235-238(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
WANG Xiang-qi, HAN Qian-qian, WANG Xuan, et al. Effect of Zhisheng Capsule on Cell Apoptosis and Fas,FasL Protein Expression in Rats Model of Esophageal Cancer[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 235-238.
DOI：

WANG Xiang-qi, HAN Qian-qian, WANG Xuan, et al. Effect of Zhisheng Capsule on Cell Apoptosis and Fas,FasL Protein Expression in Rats Model of Esophageal Cancer[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 235-238. DOI：

摘要

目的: 观察至生胶囊对食管癌造模大鼠细胞凋亡及Fas

FasL蛋白表达的影响

从分子生物学角度分析其作用机制。方法: 选用健康Wistar大鼠

除空白组外

其余大鼠均ip甲基戊基亚硝胺(MANA)5 mg·kg-1

1次/周

连续18周造模。末次注射后将造模大鼠随机分为模型组、至生胶囊高(0.75 g·kg-1)、中(0.5 g·kg-1)、低(0.25 g·kg-1)剂量组、西药替加氟组(20 mg·kg-1)

1次/d

连续4周。原位末端标记法(TUNEL)检测对食管癌癌细胞凋亡的促进作用;免疫组化法检测大鼠食管细胞中Fas

FasL蛋白的表达。结果: 至生胶囊可明显促进食管癌细胞的凋亡

高、中、低剂量组、化疗组均促进凋亡

与模型组比较均有统计学意义(P<0.05)

高、中剂量组疗效明显优于化疗组、低剂量组(P<0.05)

且呈一定量效关系。各药物治疗组Fas表达阳性率均有不同程度的升高

FasL表达阳性率均有不同程度的降低。结论: 至生胶囊能促进食管癌细胞凋亡

其作用机制可能与其提高Fas表达、降低FasL表达有关;至生胶囊促进食管癌细胞凋亡及调节Fas

FasL表达的作用存在一定的量效关系

至生胶囊高剂量此种作用优于替加氟。

Abstract

Objective: To observe the effect of Zhisheng Capsule on the rat model of esophageal cancer

inducing apoptosis and the impact on Fas

FasL protein expression. Method: Healthy Wistar rats were treated with MANA 5 mg·kg-1 for 18 weeks except normal group.After the last injection

the rats were randomly divided into the following groups:esophageal model group

Zhisheng Capsule high(0.75 g·kg-1)

middle (0.5 g·kg-1)

low (0.25 g·kg-1)dose groups

tegafur group (20 mg·kg-1). TUNEL assay was used to examine the effect of Zhisheng Capsule on apoptosis of esophageal cancer cells. Immunohisto-chemistry (IHC) technique was used to measure the expression of Fas and FasL protein in cells. Result: Zhisheng Capsule of three doses could promote apoptosis of esophageal cancer cells according to the result of TUNEL assay. Comparison with model group was significant (P<0.05). High dose group was significantly better than chemotherapy group and low dose group (P<0.05). There showed some dose-effect relationship. Measured with IHC

the expression of Fas protein in the cells dealt with Zhisheng Capsule was increased

whereas the expression of FasL protein was decreased . Conclusion: Zhisheng Capsule could promote apoptosis of esophageal cancer cells. The molecular mechanism of Zhisheng Capsule may be related with up-regulating of Fas and down-regulating the expression of FasL. In addition

the effects of Zhisheng Capsule to promote apoptosis of esophageal cancer cells and Fas

FasL showed some dose-effect relationship. The effect of the capsule was better than tegafur.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Stem-leave Total Flavonoid on Fas and FasL Expression and Anti-oxidation in Hippocampal Neurons of Rats Induced by Focal Ischemia Reperfusion

Shaoyaotang Containing Serum Mediates Fas/FasL Pathway to Inhibit Lipopolysaccharide Induced Inflammation and Apoptosis of Caco-2 Cells

Hei Xiaoyaosan Modulates Oxidative Stress and Apoptosis to Exert Neuroprotective Effect in Alzheimer's Disease Rats

Jujuboside A Improves Cognitive Function in Rat Model of VCI via PI3K/Akt Signaling Pathway

Effect of Serum Containing Zhenwutang on Apoptosis of Myocardial Mast Cells and Mitochondrial Autophagy

Related Author

CHEN Meng

ZHAO Shu-min

LI Han

KONG Xiang-yu

ZHENG Xiao-ying

XIE Nianjia

CHEN Daguang

LYU Zhaowen

Related Institution

The First Hospital of Hunan University of Chinese Medicine

People's Hospital of Ningxiang City，Ningxiang

Gansu University of Chinese Medicine

Gansu Key Laboratory of Traditional Chinese Medicine（TCM）Excavation and Innovative Transformation

Gansu Engineering Laboratory for New Products of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰