Establisnment of Method of Vancomycin in TDM and Study of Stablility

LUO Yi; LIU Wen; DENG Nan; DAI Ying-chun

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Establisnment of Method of Vancomycin in TDM and Study of Stablility

更新时间：2024-01-04

- Establisnment of Method of Vancomycin in TDM and Study of Stablility
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 20, Pages: 133-135(2011)
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- Published：2011
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LUO Yi, LIU Wen, DENG Nan, et al. Establisnment of Method of Vancomycin in TDM and Study of Stablility[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 133-135.
DOI：

LUO Yi, LIU Wen, DENG Nan, et al. Establisnment of Method of Vancomycin in TDM and Study of Stablility[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 133-135. DOI：

摘要

目的: 建立一快速、准确的RP-HPLC方法测定人血清中万古霉素血药浓度

并考察方法的稳定性。方法: 以甲硝唑为内标

采用Agilent 1100系列高效液相色谱仪

以Kromasil C18柱(4.6 mm×250 mm

5 μm)为分析柱;流动相为乙腈-0.01 mol·L-1KH2PO4溶液(7 ∶93);流速1.0 mL·min-1;检测波长236 nm;柱温30 ℃。血浆样品用30%的硫酸锌溶液沉淀蛋白后

取上清液 20 μL进样分析

对血样处理前后及标准溶液保存不同时间后的稳定性进行了考察。结果: 色谱峰分离良好

无干扰。万古霉素最低检测浓度为0.252 4 mg·L-1

线性范围0.504 8~126.2 mg·L-1

线性方程为 Y =-1.94×10-2 X +0.653( r =0.999 3)

相对回收率大于95%

高、中、低3个浓度的样品萃取回收率均大于78%

血样未经处理前4 ℃条件下能在1周内保持较好稳定性

血样处理后48 h内样品能保持较好稳定性

而标准溶液有效保存期为6个月。结论: 本方法是一种可靠、快速灵敏的检测方法

适用于万古霉素的血药浓度监测。

Abstract

Objective: To develop a HPLC method to determine the concentration of vancomycin (VAN) in human plasma and study the stability of vancomycin in different condition. Method: The metonidazole was used as inter standard (I.S.) The analytical column was a Kromasil C18 column connected with the Agilent 1100 HPLC system. The isocratic mobile phase was the acetonitrile and buffer of KH2PO4 (0.01 mol·L-1) with the rate of (7 ∶93). The detective UV wavelength was 236 nm and the column temperature was 30 ℃. The plasma samples

added metonidazole as internal standard

were deposited down the protein with 30% ZnSO4 and 20 μL of the upper phase was injected into the sampler.The stability of the samples in different conditon were studied. Result: The chromatography was good and not interfered by the components of the plasma. The linear equation was Y=-1.94×10-2X+0.653(r=0.999 3). The recovery of vancomycin in different concention were all >78%. The samples were stability in 4 ℃ for a week

the standard solution of vancomycin were stablity for a half year. Conclusion: This is a accurate

sensitive and conventient method for the dertermination of VAN in the therapeutic drug monitoring.

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