Protect Effect of Combinded Therapy of Herbal and Benazepril onEarly Stage of Diabetic Nephropathy in Rats
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Protect Effect of Combinded Therapy of Herbal and Benazepril onEarly Stage of Diabetic Nephropathy in Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 12, Pages: 202-206(2011)
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Published:2011
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HUANG Bao-ying, LIN Ying-hua, FU Xian-guo, et al. Protect Effect of Combinded Therapy of Herbal and Benazepril onEarly Stage of Diabetic Nephropathy in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 202-206.
DOI:
HUANG Bao-ying, LIN Ying-hua, FU Xian-guo, et al. Protect Effect of Combinded Therapy of Herbal and Benazepril onEarly Stage of Diabetic Nephropathy in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 202-206.DOI:
Protect Effect of Combinded Therapy of Herbal and Benazepril onEarly Stage of Diabetic Nephropathy in Rats
Objective: To study the effect and mechanism of combined Chinese and Western medicines in early diabetic nephropathy (DN) in rats. Method: The male wistar rats were randomly divided into 2 groups:normal and DN groups. Experimental DN was induced in DN group with streptozotocin (STZ)
and the normal group was given equal volume of distilled water. Then the modeled rats were subrandominzed into model group and benazepril (5 mg ·kg-1)+herbal group (50 g ·kg-1). Benazepril+herbal group was medicated respectly
the other two groups were given distilled water. After the rats had been treated for 8 weeks
transforming growth factor-β1(TGF-β1)
collagen Ⅳ
angiotensinⅡ(AngⅡ)
monocyte/macrophage(ED-1) in the renal tissues of the rats were detected by immunohistocytochemistry. Result: Compared with model group
the thickened glomerular basement membrane
increased mesangial cells
proliferated mesangialmatrix
and enlarged mesangial area became lightened in benazepril+herbal group after treatment.Moreover
TGF-β1
collagen Ⅳ
AngⅡ and ED-1 were reduced when compare to model group
but which were higher than normal group. Conclusion: This recipe combined with benazepril can reduce the pathological damage of early DN and postphone the development of DN.