Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart
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Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 17, Pages: 233-237(2011)
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Published:2011
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JIANG Jie-jun, ZHOU Jing, MA Hong-yue, et al. Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 233-237.
DOI:
JIANG Jie-jun, ZHOU Jing, MA Hong-yue, et al. Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 233-237.DOI:
Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart
Objective: To study toad venom(BV)-induced cardiac toxicity and the toxic substances in isolated guinea-pig heart. Method: Isolated guinea pig hearts were switched to the Langendorff mode and perfused with or without BV.Measure the content of toad venom in perfused hearts using HPLC. Result: BV caused auriculo-ventricular block
supraventicular tachycardia
ventricular tachycardia
ventricular fibrillation and other arrhythmias phenomenon in isolated hearts.The cumulative doses of bufadienolides (60±11.5)μg induced cardiac arrest.Nine kinds of bufadienolides were detected by HPLC in the isolated hearts.Compare their affinity with the hearts:arenobufagin>bufalin>telocinobufagin>hellebrigenin>gamabufotalin>cinobufalin>bufotalin>resibufogenin>desacety-bufotalin. Conclusion: BV has cardiotoxicity on the guinea pig heart and the material basis of toad induced toxic effects may be gamabufotalin
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