Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC

LIU Bo; ZHANG Hao-ke; GONG Hai-yan

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Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC

更新时间：2024-01-04

- Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 14, Pages: 92-94(2011)
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- Published：2011
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LIU Bo, ZHANG Hao-ke, GONG Hai-yan. Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 92-94.
DOI：

LIU Bo, ZHANG Hao-ke, GONG Hai-yan. Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 92-94. DOI：

摘要

目的: 采用反相高效液相法测定新疆枸杞叶茶中的芦丁和槲皮素的含量。方法: Kromasil C18色谱柱(4.6 mm ×250 mm

5 μm)

流动相甲醇-0.04%磷酸水溶液(用三乙胺调pH 3.0)(45 ∶55)

检测波长360 nm

流速1.0 mL ·min-1。结果: 芦丁和槲皮素的线性范围分别在0.23~1.84 μg和0.03~0.24 μg

回收率分别为98.19%

97.98%。RSD分别为1.49%

2.25%。结论: 本方法简便、可靠、重现性较好

为新疆枸杞叶茶的质量控制提供了快速、准确的测定方法。

Abstract

Objective: To determine active constituents quercetin and rutin in tea of Lycium barbarum leaves by RP-HPLC. Method: Rutin and quercertin were separated on an Kromasil C18 column (4.6 mm×250 mm

5 μm) with methanol-0.04% phosphoric acid (pH was adjusted to 3 with diethylamide) (45 ∶55) as mobile phases. The detecting wavelength was at 360 nm. The flow rate was 1.0 mL ·min-1. Result: The linear ranges of rutin and quercertin were within 0.23-1.84 μg and 0.03-0.24 μg respectively. The recoveries were 98.19% and 97.98%

RSDs were 1.49% and 2.25% respectively. Conclusion: The method is simple

accurate

and reliable

which provides the basis of a better development for the tea of L. barbarum in Xinjiang.

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Related Institution

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Xiamen Institute of Medicine,Xiamen Institute for Drug Control

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