Effects of External Auxin on Effective Components and Endogenous Hormones Contents in  Suspension Cell

HU Guo-qiang; CHEN Shun-qin; YUAN Yuan; WU Chong; LIN Shu-fang

您当前的位置：

首页 >

文章列表页 >

Effects of External Auxin on Effective Components and Endogenous Hormones Contents in Suspension Cell

更新时间：2024-01-04

- Effects of External Auxin on Effective Components and Endogenous Hormones Contents in Suspension Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 17, Pages: 127-130(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
HU Guo-qiang, CHEN Shun-qin, YUAN Yuan, et al. Effects of External Auxin on Effective Components and Endogenous Hormones Contents in Suspension Cell[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 127-130.
DOI：

HU Guo-qiang, CHEN Shun-qin, YUAN Yuan, et al. Effects of External Auxin on Effective Components and Endogenous Hormones Contents in Suspension Cell[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 127-130. DOI：

摘要

目的: 探讨外源生长素对黄芩悬浮细胞有效成分和内源激素含量的影响。方法: 将稳定继代的黄芩愈伤组织在含有不同浓度NAA或KT的MS液体培养基悬浮震荡培养

光照16 h黑暗8 h进行培养18 d后

采用HPLC的方法

测定黄芩苷的含量

采用酶联免疫吸附法测定内源激素的含量

以黑暗条件为对照。结果: ①与对照组相比

光照和黑暗条件下分别加入0.2 mg ·L-1 NAA后

黄芩苷含量显著降低

分别为未加入NAA时的2.56%

3.36%。②黑暗条件下

加入外源NAA后

ZR含量显著降低

但IAA

ABA和GAs含量变化不明显。③0.2 mg ·L-1 NAA和不同浓度KT的协同作用对黄芩苷的含量呈现先增长后降低的趋势。其中1.0 mg ·L-1的KT作用下黄芩苷含量最高

且黑暗条件下显著高于光照条件

达到2.13μg ·L-1。结论: 外源生长素不利于黄芩有效成分黄芩苷的积累

0.2 mg ·L-1 NAA+1.0 mg ·L-1 KT的组合对黄芩苷的积累量具有显著的促进作用。

Abstract

Objective: To study the effects of auxin on the active compounds and endogenous hormones in Scutellaria baicalensis suspension cell cultures. Method: The subculture scutellariae callus were cultivated in MS liquid medium containing various concentration of NAA or KT on a gyratory shaker with a 16-h photoperiod. After 18 d

the contents of baicalin and endogenous hormones were determined with HPLC and enzyme-linked immunosorbent assay

respectively. Darkness condition was as control. Result: (1) After 0.2 mg ·L-1 NAA treatment

the content of baicalin was 2.56% and 3.36% lower than of untreatment

respectively. (2) After NAA treatment on the dark conditions

the content of ZR was significantly reduced. (3) The synergistic effect of 0.2 mg ·L-1 NAA with different concentration of KT on the content of baicalin presented the trend of rising then declined. And after 1.0 mg ·L-1 KT treatment

the content of baicalin was the highest levels on the dark conditions and significantly higher than light condition. Conclusion: Auxin is not suitable for the active compound production of S. baicalensis. The combination of 0.2 mg ·L-1 NAA+1.0 mg ·L-1 KT promotes the accumulation of baicalin.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Two Kinds of Compound Rhizosphere Growth-promoting Bacteria on Growth，Physiological Characteristics and Pharmacodynamic Components of Angelicae Sinensis Radix

Quantitative Classification of Murraya paniculata Leaf Traits and Endogenous Hormone Contents Analysis

Research Progress on G₂/M Arrest in Breast Cancer Cells by Active Compounds of Traditional Chinese Medicine

Effect of Exogenous Nitric Oxide on Plant Hormones and Enzyme Content of Acanthopanax senticosus Seeds

Effect of Spraying Nano-calcium Carbonate and Calcium Nitrate Tetrahydrate on Characters， Carbohydrate Components and Endogenous Hormones of Dendrobium officinale

Related Author

YAO Yang-yang

YANG Tao

WANG Yin-quan

PENG Tong

WANG Zhi-ye

YANG Hui

Xu-tao XIE

Zhong-yi HUA

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Gansu Key Laboratory of Microbial Resources Development and Utilization，Institute of Biology， Gansu Academy of Sciences

Northwest Collaborative Innovation Center of Traditional Chinese Medicine （TCM） and Tibetan Medicine， Gansu Key Laboratory Breeding Base for TCM Quality and Standard Research

Guangxi University of Chinese Medicine

National Resource Center for Chinese Meteria Medica China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰