Astragaloside and Astragalan Determination in Angelicae Sinensis Decoction for Supplementing Blood Granules

TU Bo; WANG Zhi-yong

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Astragaloside and Astragalan Determination in Angelicae Sinensis Decoction for Supplementing Blood Granules

更新时间：2024-01-04

- Astragaloside and Astragalan Determination in Angelicae Sinensis Decoction for Supplementing Blood Granules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 18, Pages: 115-117(2011)
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- Published：2011
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TU Bo, WANG Zhi-yong. Astragaloside and Astragalan Determination in Angelicae Sinensis Decoction for Supplementing Blood Granules[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 115-117.
DOI：

TU Bo, WANG Zhi-yong. Astragaloside and Astragalan Determination in Angelicae Sinensis Decoction for Supplementing Blood Granules[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 115-117. DOI：

摘要

目的: 探讨当归补血汤颗粒中黄芪甲苷和黄芪多糖的含量测定方法。方法: 采用高效液相色谱法测定黄芪甲苷的含量

采用苯酚-硫酸比色法测定黄芪多糖的含量

旨在为自制的当归补血汤颗粒质量评价奠定基础。结果: 黄芪甲苷和黄芪多糖的平均回收率分别为97.64%

97.61%

RSD分别为2.10%

3.20%。结论: 采用高效液相色谱法测定黄芪甲苷的含量

高效液相色谱法能实现黄芪甲苷与有关物质的有效分离

黄芪甲苷峰形好

含量测定结果重复性好;所用测定黄芪多糖含量的苯酚-硫酸比色法

具有准确可靠、灵敏度高、重现性好等优点

可用于评价当归补血汤颗粒质量的有效方法。

Abstract

Objective: To investigate astragaloside and astragalan determination method in angelicae sinensis decoction for supplementing blood granules. Method: This article used HPLC to determine the content of astragaloside

and used phenol-sulfuric colorimetric method to determine the content of astragalan. Result: The average recoveries of astragaloside and astragalan were 97.64%

97.68%. Relative standard deviation was 2.10%

3.07%. Conclusion: HPLC had been used in determining the content of astragaloside and it could get an effective separation

good peak shape and reproducible assay results. Phenol-sulfuric colorimetric method was used to determine the content of astragalan

and it was accurate

reliable

high sensitivity

and good reproducibility. So they can be effectively used to evaluate the quality of angelicae sinensis decoction for supplementing blood granules.

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Related Institution

Nanjing University of Traditional Chinese Medicine（TCM）

Nanjing TCM Hospital Affiliated to Nanjing University of TCM

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