Separation,Purification and Determination of Protein in

WAN Zhi-qiang; YAN Ming-ming; ZHAN Yue; SHAO Shuai; YU Lei; YU Hong-wei; ZHAO Da-qing

您当前的位置：

首页 >

文章列表页 >

Separation,Purification and Determination of Protein in

更新时间：2024-01-04

- Separation,Purification and Determination of Protein in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 17, Pages: 116-118(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
WAN Zhi-qiang, YAN Ming-ming, ZHAN Yue, et al. Separation,Purification and Determination of Protein in [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 116-118.
DOI：

WAN Zhi-qiang, YAN Ming-ming, ZHAN Yue, et al. Separation,Purification and Determination of Protein in [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 116-118. DOI：

摘要

目的: 采用3种方法对五味子进行蛋白质提取

寻找一种简单有效的五味子蛋白提取方法。方法: 利用SDS-聚丙烯酰胺凝胶电泳法对蛋白提取物进行分析

同时依据Lowry法对五味子总蛋白含量进行测定

HPLC测定五味子蛋白提取物中氨基酸的含量。结果: 3种方法提取总蛋白质量分数分别为58.44%

19.21%

26.18%

其所含氨基酸质量分数分别为57.46%

15.41%

23.14%。结论: 采用脱脂硫酸铵沉降法提取所得总蛋白及其所含氨基酸含量较高、纯度较合理

方法较可行。

Abstract

Objective: To develop a simple method for effectively extracting protein from schisandra with the comparison of three extractive methods. Method: The extracts of the three methods were determined with SDS-PAGE

the content of protein was determined with the Lowry method

and a HPLC method was established for the determination of the amino acids in schisandra extract. Result: The protein content of the three methods was 58.44%

19.21% and 26.18%.The amino acids content of the three methods was 57.46%

15.41% and 23.14%. Conclusion: Purified protein and its amino acids were obtained by defatted ammonium sulfate precipitation procedure

and the content of protein was acceptable.This method is easily and more feasible.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Current Situation， Problems and Countermeasures of Experimental Research on Traditional Chinese Medicine Regulating PI3K/Akt Signaling Pathway in Rats with Polycystic Ovary Syndrome

Effect of Aidi Injection on Expression of Cytochrome P450 Enzyme in DEN-induced Hepatocellular Carcinoma Rats

Pre-column Derivatization RP-HPLC for Simultaneous Determination of 17 Amino Acids in Cynomorii Herba from Different Producing Areas and Its Multivariate Statistical Analysis

Determination of Jujuboside A and Schisandrin Contents before and after the Combination of Semen Ziziphi Spinosae with Schisandra Chinensis

Related Author

YAN Pengxuan

LIU Yiqing

XIAN Nanxing

PENG Linjing

LI Kun

ZHANG Jingchun

ZHAO Yukun

Xiao-qing ZHU

Related Institution

Institute of Basic Theory for Chinese Medicine，China Academy of Chinese Medical Sciences

Xiyuan Hospital， China Academy of Chinese Medical Sciences

China Academy of Chinese Medical Sciences

State Key Laboratory of Functions and Applications of Medicinal Plants， Guizhou Provincial Key Laboratory of Pharmaceutics， Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine （Ministry of Education）， School of Pharmacy， Guizhou Medical University

College of Pharmacy, Gansu University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰