Differences in Sensitivity of Passive Cutaneous Anaphylaxis Induced by Breviscapine Among Three Rat Strains
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Differences in Sensitivity of Passive Cutaneous Anaphylaxis Induced by Breviscapine Among Three Rat Strains
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 9, Pages: 169-172(2011)
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Published:2011
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TAN Meng-hui, LI Xiu-fang, JIN Ruo-min, et al. Differences in Sensitivity of Passive Cutaneous Anaphylaxis Induced by Breviscapine Among Three Rat Strains[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 169-172.
DOI:
TAN Meng-hui, LI Xiu-fang, JIN Ruo-min, et al. Differences in Sensitivity of Passive Cutaneous Anaphylaxis Induced by Breviscapine Among Three Rat Strains[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 169-172.DOI:
Differences in Sensitivity of Passive Cutaneous Anaphylaxis Induced by Breviscapine Among Three Rat Strains
Objective: To compare the differences in sensitivity of passive cutaneous anaphylaxis (PCA) caused by breviscapine among Sprague Dawley (SD)
Wistar
and Fischer344(F344) rats. Method: The rats in the 3 strains were randomly divided into negative control group
trichosanthin group and breviscapine group. The homologous PCA experiment was performed by using three different strains of rat and xenogenic PCA by using rats and mice. Breviscapine was given as allergen. The absorbance was measured to discriminate the severity of PCA. Result: The rat homologous PCA revealed that after injection with 25 mg · kg-1 breviscapine
F344 rat showed positive reaction
the absorbance was 0.064±0.005 7 and increased significantly (P<0.05)
but SD rat and Wistar rat showed negative reaction. Xenogeneic PCA showed that the mice injected breviscapine antiserum of F344 rat
demonstrated a positive reaction (P<0.05)
the absorbance was 0.10±0.01
the others received breviscapine antiserum of SD or Wistar rats showed negative reactions. Conclusion: There are differences in sensitivity to breviscapine among three rat strains