Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology
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Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 11, Pages: 7-12(2011)
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Published:2011
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GUO Kai, liangjianping, hualanying, et al. Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 7-12.
DOI:
GUO Kai, liangjianping, hualanying, et al. Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 7-12.DOI:
Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology
The objective of the current study was to determine the optimum ultrasonic extraction conditions for hypericin from Hypericum perforatum L. The optimum conditions were evaluated with 2-level fractional factorial design and optimized using response surface methodology. High yields of hypericin were observed from Hypericum perforatum L. by ultrasonic extraction technology. The extraction time
acetone concentration and the ratio of solvent to material were the most significant parameters on hypericin extraction as evaluated through FFD. The extraction conditions were further investigated with central composite design. The results showed that the optimal parameters for this method were: extraction time of 36.15 min
acetone concentration of 68.14(Vol) of acetone/ethanol solvent
and ratio of solvent to material of 11.87 mL?g-1. Under the optimized condition
the maximum productivity of hypericin is 1.44%. The yield of hypericin under the optimized extraction conditions were great higher than that obtained from conventional solvent extraction. The present study demonstrates that ultrasonic is a great efficient tool for the fast extraction of hypericin from Hypericum perforatum L.
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