Objective: To study the impacts of culture conditions on the Caco-2 cell model

to optimize the appropriate conditions for a stable

reproducible Caco-2 model. Mehtod: The environment of small intestine was in vitro simulated

and rat tail collagen was coated on three types of Transwell monolayerly and compared.Morphological features were observed by microscope

the integrity

polarity and permeability of monolayer were measured by the resistance

alkaline phosphatase

the amount of sodium fluorescein. Result: After culture of 21 days

the integrity

polarity of Caco-2 monolayer cultured on 24-well Transwell were higher than that on 12-well Transwell

but the permeability were the opposite.The TEER of the monolayer coated with rat tail collagen was increased rapidly in early stages

and was no significant effect at the middle and late stages. Conclusion: Three types of Transwell can be successfully used to culture Caco-2 cell monolayer.24-well Transwell is suitable for small samples with high sensitivity and high-throughput screening of a large number of samples.12-well Transwell has higher volume of sample and permeability

is suitable for routine laboratory operations and equipment.