Effects of Shugan Jianpi Recipe on 5-Hydroxytryptamine,Calcitonin Gene Related Peptide in Rats with Diarrhea-predominant Irritable Bowel Syndrome
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Effects of Shugan Jianpi Recipe on 5-Hydroxytryptamine,Calcitonin Gene Related Peptide in Rats with Diarrhea-predominant Irritable Bowel Syndrome
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 14, Pages: 179-181(2011)
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Published:2011
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LIU Jie-min, LI Hong-ling, HU Hao, et al. Effects of Shugan Jianpi Recipe on 5-Hydroxytryptamine,Calcitonin Gene Related Peptide in Rats with Diarrhea-predominant Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 179-181.
DOI:
LIU Jie-min, LI Hong-ling, HU Hao, et al. Effects of Shugan Jianpi Recipe on 5-Hydroxytryptamine,Calcitonin Gene Related Peptide in Rats with Diarrhea-predominant Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 179-181.DOI:
Effects of Shugan Jianpi Recipe on 5-Hydroxytryptamine,Calcitonin Gene Related Peptide in Rats with Diarrhea-predominant Irritable Bowel Syndrome
Objective: To observe the effect of Shugan Jianpi Recipe on 5-hydroxytryptamine(5-HT)
calcitonin gene related peptide(CGRP) in rats with diarrhea predominant irritable bowel syndrome(D-IBS). Method: Sixty Wistar rats were randomized into normal group
model group
low
middle and high-dose groups of Shugan Jianpi Recipe
and dicetel group.Cold-binding applications were given to establish similar model of D-IBS.Violet spectrophotometer was used for determining the levels of 5-HT in serum.RIA was used for measuring the levels of CGRP in serum.The expressions of 5-HT and CGRP in intestinal and colon mucosa and colon tissue were detected by immunohistochemical SABC method. Result: In the model group
the 5-HT level increased
CGRP decreased
compared with those in the normal group(P<0.05).The Shugan Jianpi Recipe's middle
high-dose increased 5-HT and decreased CGRP significantly
compared with that in the model group(P<0.01). Conclusion: The therapeutic mechanism of Shugan Jianpi Recipe for D-IBS is probably related with adjustment of 5-HT and CGRP in serum and colon tissue.
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