Experimental Study of Extract of  on Content of Hydroxyproline in Femur of Ovariectomized Rats

WANG Xiao-lan; ZHENG Xiao-ke; WEI Yue; WU Hui-min; NING Tao-li; ZHU Yan-qin

您当前的位置：

首页 >

文章列表页 >

Experimental Study of Extract of on Content of Hydroxyproline in Femur of Ovariectomized Rats

更新时间：2024-01-04

- Experimental Study of Extract of on Content of Hydroxyproline in Femur of Ovariectomized Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 10, Pages: 132-136(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011
- 稿件说明：
移动端阅览
WANG Xiao-lan, ZHENG Xiao-ke, WEI Yue, et al. Experimental Study of Extract of on Content of Hydroxyproline in Femur of Ovariectomized Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 132-136.
DOI：

WANG Xiao-lan, ZHENG Xiao-ke, WEI Yue, et al. Experimental Study of Extract of on Content of Hydroxyproline in Femur of Ovariectomized Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 132-136. DOI：

摘要

目的: 采用柱前衍生化法对去势大鼠股骨中羟脯氨酸(HyP)的含量进行测定

观察卷柏提取物对去势大鼠股骨中HyP含量的影响。方法: Wistar雌性大鼠摘除卵巢后1周开始ip卷柏总提物665 mg ·kg-1

卷柏正丁醇部位85 mg ·kg-1

卷柏水部位490 mg ·kg-1

持续11周后处死

取其股骨

采用2

4-二硝基氯苯柱前衍生化反相高效液相色谱法

观察卷柏提取物对去势大鼠股骨中HyP含量的影响。结果: HyP 0.125~2.00 g ·L-1 线性关系良好(r=0.999 4)

精密度试验RSD<1%

24 h内稳定性试验RSD<5%

加样回收率均在90%~110%;卷柏水部位和正丁醇部位均能增加去势大鼠股骨中HyP含量。结论: 卷柏提取物能增加去势大鼠股骨中HyP含量

其中卷柏水部位和正丁醇部位

对去势大鼠骨质疏松具有一定的干预作用。

Abstract

Objective: To study the effect of the extract of Selaginella tamariscina on the content of hydroxyproline in femur of ovariectomized rats. Method: Wistar rats were ovariectomized except for the normal group. One week after operation

the rats were treated with different medicines. Eleven weeks after treated the medicines

the rats were killed

the content of hydroxyproline in femur of ovariectomized rats was determined by RP- HPLC with pre-column derivatization by 2

4-dinitrochlorobenzene (DNCB). Result: Calibration curves showed good linearity within the concentration range of 0.125-2.00 g ·L-1 for hydroxyproline. The RSD of precision was less than 1%

the RSD of stability of the sample was less than 5% in 24 hours and recoveries of assay were 90%-110%. The water part group and n-butanol part group of S

tamariscina could increase the content of hydroxyproline in femur of ovariectomized rats. Conclusion: The extract of S. tamariscina can increase the content of hydroxyproline in femur of ovariectomized rats

suggesting that the water part group and n-butanol part group of S.tamariscina can intervene the osteoporosis in ovariectomized rats.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Different Administration Methods with Bleomycin on Pulmonary Fibrosis in Rats

Experimental study on Preventive Effect of Weitong Injection on Postoperative Adhesion in Rats

Determination of Emodin and Chrysophanol in Qingshenjianfei Tablets by RP-HPLC

Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC

Related Author

He WANG

Guang-ping ZHANG

Hong-ping HOU

Yun-hang GAO

Shuang-rong GAO

Meng MA

Hai-jing ZHANG

Zhong-xiu ZHANG

Related Institution

Guiyang University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Ethnic Pharmaceutical Institute of Southwest University for Nationalities

Gannan Medical University

Guangxi Traditional Chinese Medicine University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰