Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 14, Pages: 73-75(2011)
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Published:2011
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JI Xiao-jun, ZHAO Si-jun, ZHANG Fu-sheng, et al. Determination of Gastrodin in Mateng Zhixuan San[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 73-75.
DOI:
JI Xiao-jun, ZHAO Si-jun, ZHANG Fu-sheng, et al. Determination of Gastrodin in Mateng Zhixuan San[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 73-75.DOI:
流速1.0 mL ·min-1。 结果 :天麻素对照品进样量在0.1~0.8 μg内呈线性关系(r=0.999 8)
稳定性试验RSD 0.94%
精密度试验RSD 0.76%
重复性试验RSD 1.1%
平均回收率为98.1%
RSD 1.01%。 结论 :该法具有操作简便、灵敏度高、重现性好等特点
可作为麻藤止眩散的质量控制方法。
Abstract
Objective: To determine the content of gastrodin in Mateng Zhixuan San by HPLC. Method : High performance liquid chromatography (HPLC) was appatiecl to determinae gastrodin content in Mateng Zhixuan San on RP C18 bonded with silica gel column with acetonitrile-0.1% phosphoric acid water (3 ∶97) as mobile phase
detection wavelength 220 nm
flow rate was 1.0 mL ·min-1. Result : Gastrodin was linear in the range of 0.1-0.8 μg(r=0.999 8)
RSD was 0.94%
precision test RSD was 0.76% and reproducibility test RSD was 1.1%. The average recovery was 98.1% and RSD was1.01%(n=6). Conclusion :The method is simple and sensitive for the quality control of this preparation.