Simultaneous Determination of Shanzhiside Methylester by HPLC in Wild and Cultivated of Qinghai

JIN Lan; LUO Gui-hua

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Simultaneous Determination of Shanzhiside Methylester by HPLC in Wild and Cultivated of Qinghai

更新时间：2024-01-04

- Simultaneous Determination of Shanzhiside Methylester by HPLC in Wild and Cultivated of Qinghai
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 14, Pages: 71-73(2011)
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- Published：2011
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JIN Lan, LUO Gui-hua. Simultaneous Determination of Shanzhiside Methylester by HPLC in Wild and Cultivated of Qinghai[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 71-73.
DOI：

JIN Lan, LUO Gui-hua. Simultaneous Determination of Shanzhiside Methylester by HPLC in Wild and Cultivated of Qinghai[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 71-73. DOI：

摘要

目的: 对野生和栽培藏药独一味中山栀苷甲酯成分进行高效液相色谱的含量测定。方法 :采用色谱柱WelchromTM Kromasil C18 (4.6 mm×250 mm

5 μm)

流动相为乙腈(A)-水(B)梯度洗脱(0~11 min

9％A;11~35 min

9％~18％A;35~45 min

18％A)

流速1.0 mL ·min-1

检测波长235 nm

柱温为25 ℃。结果 :山栀苷甲酯的线性范围0.25~2.12 mg (r=0.999 8);平均回收率为100.7%

RSD为0.89％。结论 :野生和栽培独一味的山栀苷甲酯的含量分别为1.81

0.75 mg ·g-1。

Abstract

Objective: To determinate the content of shanzhiside methylester in wild and cultivated Lamiophlomis rotate by HPLC. Method :The samples were separated on an WelchromTM Kromasil C18 (4.60 mm×250 mm

5 μm) with acetonitrile(A)- water(B)(0-11 min

9％ A;11-35 min

9％-18％ A;35-45 min

18％ A) colnmu as the mobile phase at a flow rate of 1.0 mL ·min-1. The effluent was monitored at 235 nm and column temperature at 30 ℃ . Result : The linear ranges of shanzhiside methylester was 0.25-2.12 mg ·g-1 (r=0.999 8). The average recoverigs were 100.7％. Conclusion :The contents of shanzhiside methylester in wild and cultivated Lamiophlomis rotate was 1.81

0.75 mg ·g-1.

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