Effects of Guizhitang and It’s Biotransformation Product on Catabolizing Enzymes of Prostaglandin Ein bEnd.3 with Interleukin- 1 β Inducement

CHEN Bing; BAI Dong; LI Cang-hai; ZHANG Chang-bin; NIU Xiao-hong; LI Lan-fang; GUO Shu-ying; SONG Jian-nan; JIANG Ting-liang

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Effects of Guizhitang and It’s Biotransformation Product on Catabolizing Enzymes of Prostaglandin Ein bEnd.3 with Interleukin- 1 β Inducement

更新时间：2024-01-04

- Effects of Guizhitang and It’s Biotransformation Product on Catabolizing Enzymes of Prostaglandin Ein bEnd.3 with Interleukin- 1 β Inducement
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 16, Pages: 128-132(2011)
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- Published：2011
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CHEN Bing, BAI Dong, LI Cang-hai, et al. Effects of Guizhitang and It’s Biotransformation Product on Catabolizing Enzymes of Prostaglandin Ein bEnd.3 with Interleukin- 1 β Inducement[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 128-132.
DOI：

CHEN Bing, BAI Dong, LI Cang-hai, et al. Effects of Guizhitang and It’s Biotransformation Product on Catabolizing Enzymes of Prostaglandin Ein bEnd.3 with Interleukin- 1 β Inducement[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 128-132. DOI：

摘要

目的: 研究桂枝汤及其生物转化模拟产物对白细胞介素-1β(IL-1 β)介导的小鼠脑微血管内皮细胞株(bEnd.3)细胞前列腺素E2(PGE2)代谢通路的影响。方法: 制备了桂枝汤及桂枝汤生物转化模拟产物的含药血清(分别设五个浓度组)

采用酶联免疫吸附法(ELISA)和免疫印记法(Western blot)

观察桂枝汤及其生物转化模拟产物对IL-1 β 刺激的bEnd.3细胞磷酸化丝裂原活化的蛋白激酶p38(p-p38MAPK)、胞浆型磷脂酶A2(cPLA2)、分泌型磷脂酶A2(sPLA2)、环氧合酶2(COX-2)、前列腺素E合成酶(PGES)、15-羟基前列腺素脱氢酶 (15-PGDH)、磷酸化核因子κB (p-NF-κB)以及前列腺素E2 (PGE2)分泌及表达的影响。结果: IL-1 β 作用于细胞10 h后

各项指标均明显上调

与对照组比较

P<0.01。除质量终浓度为2.5%的桂枝汤含药血清对PGES

sPLA2;终浓度为1%的模拟药对PGES;终浓度为2.5%的含药血清对PGES

sPLA2

cPLA2作用不显著外

其他各浓度组与模型组比较均出现显著性差异。结论: 桂枝汤及其生物转化模拟产物对IL-1 β 刺激bEnd.3细胞合成PGE2的代谢酶系统有不同程度的抑制作用

从而降低了前炎症介质和中枢发热介质PGE2的释放和分泌。结果提示

桂枝汤及其生物转化模拟产物对脑微血管内皮细胞分泌PGE2具有相似的药效

为模拟产物的应用和开发提供了实验支持。

Abstract

Objective: To investigate the effects of Guizhitang and it’s biotransformation product on catabolizing enzymes of prostaglandin E2(PGE2) in bEnd.3 with IL-1β inducement. Method: Using Elisa and Western blot methods to measure p-p38MAPK

cPLA2

sPLA2

COX-2

PGES

15-PGDH

p-NF-κB and PGE2. Result: Guizhitang and it’s biotransformation product could inhibit the changes in p-p38MAPK

cPLA2

sPLA2

COX-2

PGES

15-PGDH

p-NF-κB and PGE2 in bEnd.3 with IL-1 β inducement. Conclusion: Guizhitang and it’s biotransformation product could inhibit the changing on catabolizing enzymes of PGE2 in bEnd.3 with IL-1β inducement to various degrees. Guizhitang and it’s biotransformation product showed similar effects. It can provide experimental evidence on application development of biotransformation product.

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Related Institution

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Shandong University of Traditional Chinese Medicine（TCM），Ji'nan

Affiliated Hospital of Shandong University of TCM，Ji'nan

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