Objective: Optimize the extract condition of rutin in Morus alba to establish a quantification method for rutin. Method : Single-factor analysis was applied to optimize the extract condition of rutin in Morus alba HPLC was used in the determination of rutin on C18 column(4.6 mm×250 mm

5 μm) with a mixture of acetonitrile-0.1% phosphoric acid (19 ∶81) as a mobile phase at the flow rate of 1.0 mL ·min-1. Rutin was detected at 360 nm

column temperature was 30 ℃. Result : The optimal extract condition was the solid-to-liquid ratio 1 ∶25

50% ethanol refluxed for 60 min. The linearity of rutin was in the range of 0.1-2.0 μg. The average recovery rate of rutin was 102.4% with RSD 1.8% (n=6). Conclusion : The determination method was simple

accurate and reliable

it could be used as the determination method of rutin in Morus alba.