Protective Effect of Saponins of on Immunological Liver Injury in Mice
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Protective Effect of Saponins of on Immunological Liver Injury in Mice
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 7, Pages: 159-162(2011)
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Published:2011
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WANG Yong-zhong, JIANG Hui, ZHANG Jia-fu, et al. Protective Effect of Saponins of on Immunological Liver Injury in Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 159-162.
DOI:
WANG Yong-zhong, JIANG Hui, ZHANG Jia-fu, et al. Protective Effect of Saponins of on Immunological Liver Injury in Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 159-162.DOI:
Protective Effect of Saponins of on Immunological Liver Injury in Mice
Objective: To observe the protective action of Panax notoginseng saponins (PNS)on immunological liver injury in mice. Method: Mice were randomly divided into five groups
control group
model group
low-dose (100 mg ·kg-1) of PNS
middle-dose (200 mg ·kg-1) of PNS
high-dose (400 mg ·kg-1) of PNS and bifendate (150 mg ·kg-1) group. The model of mouse with immunological liver injury was induced by injection of BCG and LPS from tail vein. The ALT
AST
MDA content
SOD and GSH-Px activities were assayed by spectrophotometry
hepatic tissues stained with hematoxylin and eosin (HE) were prepared for histological analysis to liver injury. Moreover TNF-α mRNA expression in liver tissue was detected by RT-PCR technology. Result: PNS could decrease the enlarged liver
spleen index
ALT
AST and MDA content(P<0.05 or P<0.01)
prevented the reduction of SOD and GSH-Px activities in liver homogenate(P<0.05 or P<0.01). Meanwhile
the pathological injury of hepatic tissues was alleviated and the expression of TNF-α mRNA in liver tissue was inhibited. Conclusion: PNS showed significant protective action on immunological liver injury in mice.
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