Effect of Crotonis Fructus Alkaloid on Apoptosis of Human Hepatoma SMMC-7721 Cells and Bcl-2/Bax Expression

CHEN Wu; CHEN Peng-ying; LIU Peng; LIAO Yan-lin

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Effect of Crotonis Fructus Alkaloid on Apoptosis of Human Hepatoma SMMC-7721 Cells and Bcl-2/Bax Expression

更新时间：2024-01-04

- Effect of Crotonis Fructus Alkaloid on Apoptosis of Human Hepatoma SMMC-7721 Cells and Bcl-2/Bax Expression
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 11, Pages: 199-201(2011)
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- Published：2011
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CHEN Wu, CHEN Peng-ying, LIU Peng, et al. Effect of Crotonis Fructus Alkaloid on Apoptosis of Human Hepatoma SMMC-7721 Cells and Bcl-2/Bax Expression[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 199-201.
DOI：

CHEN Wu, CHEN Peng-ying, LIU Peng, et al. Effect of Crotonis Fructus Alkaloid on Apoptosis of Human Hepatoma SMMC-7721 Cells and Bcl-2/Bax Expression[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 199-201. DOI：

摘要

目的: 研究巴豆生物碱(croton seed alkaloid

CA)对人肝癌SMMC-7721细胞的诱导凋亡作用并探讨其对凋亡相关蛋白Bax和Bcl-2表达的影响。方法: 不同浓度CA分别处理SMMC-7721细胞24~48 h后

MTT法检测细胞的生长活性

流式细胞仪检测细胞凋亡率

Western blot检测凋亡相关蛋白 Bax

Bcl-2的表达。结果: 10~200 mg ·L-1 CA均能抑制SMMC-7721细胞增殖(P<0.05)

其抑制率与作用时间及浓度相关;CA作用SMMC-7721细胞24 h后

对照组凋亡率为(0.26±0.14)%

100

200 mg ·L-1CA组细胞凋亡率分别为(5.85±0.47)%

(17.70±1.23)%

(33.25±2.08)%

(49.52±1.85)%

与对照组比较

差异有统计学意义(P＜0.05);Western blot结果提示经CA作用24 h后

随着药物浓度的增加

Bax的表达逐渐增加

而 Bcl-2的表达逐渐减少。结论: CA能抑制 SMMC-7721细胞的生长并促进其凋亡

其发生与Bcl-2蛋白的减少和Bax蛋白表达增多有关。

Abstract

Objective: To study the effect of Crotonis Fructus alkaloid (CA) on apoptosis and the expression of Bcl-2 and Bax in human hepatoma SMMC-7721 cells. Method: After treatment with CA for 24-48 h

MTT assay was used to determine the cell growth inhibitory rate in vitro

flow cytometry was used to detect the apoptosis of SMMC-7721 cells before and after CA treatment.Western blot was used to detect the expression of Bcl-2 and Bax. Result: CA inhibited the growth of SMMC-7721 cells in a dose-and time-dependent manner.After treatment with 50-200 mg ·L-1 CA for 24 h

the number of apoptosis cells was increased dose-dependently from (0.26±0.14)% in the control group to (5.85±0.47)%

(17.70±1.23)%

(33.25±2.08)% and (49.52±1.85)% in CA groups

respectively.By Western blot analysis

we found the level of Bax was up-regulated

whereas

the level of Bcl-2 down-regulated. Conclusion: CA can efficiently inhibit the proliferation and induce apoptosis in SMMC-7721 cells.Its mechanism is likely related to the down-regulation of Bcl-2 and up-regulation of Bax.

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