Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC
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Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 12, Pages: 70-71(2011)
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Published:2011
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WANG Wei-ming, HUO Jin-hai, SUN Guo-dong. Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 70-71.
DOI:
WANG Wei-ming, HUO Jin-hai, SUN Guo-dong. Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 70-71.DOI:
Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC
Objective: To establish the HPLC-DAD method for determination of the Hypoxanthine in Qinbaiqingfeinongsuo pills. Method: The separation was performed in a Diamonsil C18 (4.6 mm×250 mm
5 μm) column with a mobile phase of methanol-water (2 ∶98) in a flow rate of 1.0 mL ·min-1 elution at 254 nm and the column temperature was 30 ℃. Result: The linear range of Hypoxanthine was 1.94-31.04 mg ·L-1(r=0.999 9). The average recovery was 99.14%
RSD 1.28%. Conclusion: This method is simple
reliable
accurate
which offers advantages of higher sensitivity and precision.It can be used for quality control of Qinbai qingfei nongsuo pills.