Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from

CHENG Yue; WANG Zhi-yu; WANG Dong-mei; LIU Hao-wen; CHEN Jian-ping

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Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from

更新时间：2024-01-04

- Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 9, Pages: 142-145(2011)
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- Published：2011
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CHENG Yue, WANG Zhi-yu, WANG Dong-mei, et al. Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 142-145.
DOI：

CHENG Yue, WANG Zhi-yu, WANG Dong-mei, et al. Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 142-145. DOI：

摘要

目的: 探讨不同溶剂对鸡血藤提取物中的总黄酮含量和抗肿瘤活性的影响。方法: 分别用不同浓度的乙醇水溶液、丙酮水溶液作为提取溶剂

利用回流法、超声提取法制备鸡血藤提取物;采用四甲基噻唑蓝(MTT)法测定提取物对结肠癌HT-29细胞增殖的抑制活性;采用分光光度法测定提取物中总黄酮含量;采用HPLC法测定提取物中芒柄花素含量。结果: 60%乙醇回流60 min所得提取物总黄酮含量最高并且对HT-29细胞增殖的抑制活性最强。结论: 鸡血藤抗肿瘤活性提取物的制备以60%乙醇回流提取为佳。

Abstract

Objective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent

different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC

respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent

refluxing as extraction method

obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell.

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