Objective: To establish an HPLC method for content determination of total scopoletin in wild and cultivated Cortex Lycii from different areas. Method: The separation was performed on a column C18

the mobile phase was acetonitrile-1% acetic acid (15 ∶85);the flow rate was 0.8 mL ·min-1;the column temperature was at 30 ℃; the detective wavelength was at 345 nm. Result: The linear range of scopoletin was from 6 to 48 mg ·L-1.The average recovery was 97.19% (RSD 1.77%). Conclusion: The method is reliable

accurate and has a good specificity.