Protection of Ganle Keli on Liver Fibrosis in Rat

JIANG Hui; SHANG Li-li; XU Song-ling; WANG Huan

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Protection of Ganle Keli on Liver Fibrosis in Rat

更新时间：2024-01-04

- Protection of Ganle Keli on Liver Fibrosis in Rat
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 12, Pages: 167-170(2011)
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- Published：2011
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JIANG Hui, SHANG Li-li, XU Song-ling, et al. Protection of Ganle Keli on Liver Fibrosis in Rat[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 167-170.
DOI：

JIANG Hui, SHANG Li-li, XU Song-ling, et al. Protection of Ganle Keli on Liver Fibrosis in Rat[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 167-170. DOI：

摘要

目的: 观察肝乐颗粒(Ganle Keli

GL)对肝纤维化大鼠的保护作用。方法: 除正常组外

其余各组采用四氯化碳诱导肝纤维化模型

每周2次

连续12周。于造模第7周起

给药组分别ig给予相应的受试药物

正常组和模型组给予等量溶媒

疗程6周。实验结束后

股动脉采血

比色法测定血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和肝匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)活性、羟脯氨酸(Hyp)含量;同时取固定部位肝脏组织

HE染色;免疫组化技术测定肝组织中核因子-κB(NF-κB)蛋白的表达

RT-PCR技术测定肿瘤坏死因子-α(TNF-α) mRNA的表达。结果: 肝乐颗粒1.5

6 g ·kg-1各剂量组能明显降低肝纤维化大鼠ALT

AST

MDA

Hyp含量

升高SOD水平;减轻大鼠肝纤维化增生程度的同时抑制肝组织中NF-κB蛋白和TNF-α mRNA的表达。结论: 肝乐颗粒对CCl4诱导的肝纤维化大鼠有很好的保护作用。

Abstract

Objective: To study the protection of Ganle Keli (GL) on liver fibrosis in rats. Method: Rat liver fibrosis was induced by CCl4 twice a week for 12 weeks. GL was used daily via lavage starting from the 7th week of CCl4application. Contents of ALT

AST

MDA

SOD and Hyp were determinate by chromatometry. Liver samples were collected after experiments

stained by HE and immuninochemistry of NF-κB. Moreover TNF-αmRNA expression in liver tissue was detected by RT-PCR technology. Result: GL could significantly reduce ALT

AST and MDA contents

increase the level of SOD. Meanwhile GL could effectively prevent the expression of NF-κB and the degree of hepatic fibrosis

and significantly reduce TNF-αmRNA in rats with liver fibrosis. Conclusion: GL has protective effect on liver fibrosis.

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Related Institution

Affiliated Hospital of Nanjing University of Chinese Medicine

School of Traditional Chinese Medicine， Jinan University

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